Labortechniker(In)

Ihre Aufgaben

  • Molekularbiologische Arbeitstechniken (GoldenGate Cloning und CRISPR/Cas9)
  • Durchführung mikrobiologischer Versuche
  • Analytische Methoden zur Quantifizierung von Metaboliten und Proteinen
  • Kultivierung von Mikroorganismen in Bioreaktoren
  • Genaue Protokollierung der Labortätigkeit
  • Unterstützung bei administrativen Tätigkeiten
  • Betreuung und Maintenance der Laborinfrastruktur

Ihr Profil

  • HTL, BSc oder MSc (Biotechnologie/Chemie/Biochemie/Molekularbiologie – FH bevorzugt), MTA, Chemotechnikerschule oder eine vergleichbare Ausbildung
  • Berufserfahrung als LabortechnikerIn von Vorteil
  • Praktische Erfahrung mit Fermentationstechniken (Bioreaktor) und / oder Molekularbiologie von Vorteil
  • Kenntnisse in Biochemie und Molekularbiologie erwünscht
  • Fähigkeit zur Integration in ein Team
  • Sehr gute Englischkenntnisse
  • EDV-Kenntnisse erwünscht
  • Selbständigkeit und Genauigkeit sind gefragt

Arbeitsort

Ort Ihrer Tätigkeit ist an der Universität für Bodenkultur, Department für Biotechnologie, Institut für Mikrobiologie und Mikrobielle Biotechnologie, Muthgasse 18, 1190 Wien

Beschäftigungsausmaß: 30-40 h / Woche

Wenn Sie interessiert, motiviert und teamfähig sind, dann freuen wir uns über Ihre aussagekräftigen Bewerbungsunterlagen per E-Mail (bitte alle Unterlagen in einem pdf Dokument) unter Angabe der Ref-Nr. 19-01103 an birgit.marckhgott(a)boku.ac.at

Bewerbungsfrist: 20. Dezember 2019

PhD and Post Doc Positions

2 PhD positions in the project “Yeast production platform for complex proteins”

Project description

Yeasts are important hosts for production of industrial enzymes and biopharmaceutical proteins. Pichia pastoris is among the most used yeasts for recombinant protein production, and strong efforts have been made over the last years to develop tools for efficient strain development. In this project, we will work on synthetic biology tools to enable rapid strain design for the production of several difficult-to-express proteins.

  • One PhD project will focus on the development, validation and implementation of a synthetic biology toolset for Pichia pastoris
     
  • The second PhD project will focus on design customized Pichia pastoris strains for several complex proteins, and the development and validation of fermentation processes for their production.

Your benefits

  • Be part of a highly interacting interdisciplinary project team
  • Be at the front of synthetic biology research with an industrially used microorganism
  • Work in collaboration with an international industrial partner
  • Start to build up your scientific network in an international team of researchers

Your requirements

  • Master’s degree or equivalent in biotechnology, molecular biology, biochemical engineering, biochemistry, or similar study
     
  • Strong practical experience in molecular biology and/or bioreactor cultivations requested, and scientific interest in synthetic biology and bioinformatics applications desired.
  • Excellent skills in spoken and written English, an organized approach with strong attention to detail and good communication skills with ability to work well in teams.

Location
Research will take place in the research groups of Prof. Diethard Mattanovich and Prof. Brigitte Gasser at the Department of Biotechnology of the University of Natural Resources and Life Sciences Vienna (BOKU). Department of Biotechnology, Institute of Microbiology and Microbial Biotechnology, Muthgasse 18, 1190 Vienna.

Start

January 2020 or later.

Interested? - Please contact

Prof. Diethard Mattanovich diethard.mattanovich@boku.ac.at

Birgit Marckhgott birgit.marckhgott(at)boku.ac.at

Bachelor or Master Thesis

Engineering the yeast Pichia pastoris to become a good brewer

Background

Saccharomyces cerevisiae and its sibling species are established as the predominant beer and wine fermenting yeasts, based on their distinct ability to ferment sugar to alcohol even in aerobic conditions. While the genetic background of this so-called Crabtree positive phenotype is well understood, there is not much known about the evolution of yeast fermentation. We have recently shown that overexpression of a single transcription factor is sufficient to turn the methylotrophic yeast Pichia pastoris into a fermenting, Crabtree positive yeast (Ata et al., Nature Commun. 2018; 9:4911). First tests have indicated that this engineered strain creates a pleasant aroma in beer and wine.

Aims

The basic strain shall be further engineered for safe use and better brewing features (gene cloning without markers by CRISPR/Cas9; overexpression of maltase). Alcohol tolerance shall be improved by adaptive evolution. Lab scale brewing and wine fermentation experiments shall evaluate the potential of the achieved strains. The overall aim is to create the first fully synthetic brewing yeast by converting a non-fermenting Crabtree negative yeast via several steps into a good brewer, thus mimicking brewer’s yeast evolution in a nutshell.  This project will be a demonstration of applying the principles of synthetic biology to an everyday question, making the concepts of biological parts and chassis easy to understand for the public.

Location

Department of Biotechnology, Institute of Microbiology and Microbial Biotechnology, Muthgasse 18, 1190 Vienna.

Duration

8-12 weeks for a bachelor thesis, 6 months for a master thesis. The content will be adapted to the duration.

Start

September 2019 or later.

Interested? - Please contact

Prof. Diethard Mattanovich diethard.mattanovich@boku.ac.at

Dr. Özge Ata oezge.ata(at)boku.ac.at


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