The BOKU Core Facility Multiscale Imaging is the successor of the BOKU-VIBT Imaging Center, the oldest core facility of the university. It was established as a pioneer research platform in 2010, offering, form the very beginning, open access to various high-end microscopes to all students, research staff and professors independent of their affiliation. Non-BOKU users, too are very welcome!
The current facility harbours eight microscopes of various kind. Although all of them are light microscopes, according to their hardware components and relevant application fields they belong to four different groups: wide-field fluorescence microscopes, confocal laser scanning microscopes, label free, chemical imaging microscopes, and a laser micro-dissection microscope is also part of the equipment. Technical details can be found in the menu: Equipment.
The facility offers training for all the microscopes, access to dedicated soft-wares for image analysis, and co-operation in research projects.
COVID-19 guidelines and safety rules
BOKU Core Facility Multiscale Imaging (prev. Imaging Center)
The BOKU Core Facility Multiscale Imaging (BCFMI) harbours numerous high-end research microscopes that are used by various departments and few external users. Therefore, the equipment of the facility is usually handled by many individual on a daily basis. To minimize the risk of SARS-CoV-2 infection, new safety rules are applicable during the current pandemic. Please, find below the COVID-19 guidelines; these must be read and approved if someone wishes to work in the BCFMI.
Before re-start, the equipment of the facility will be checked and laboratory areas will be prepared for proper operation to keep the specific safety rules.
Face masks:are mandatory when entering/leaving the facility and during the whole time of laboratory work. Scarfs and buff-sleeves can be used as alternative, in case no masks are available. Users must wear their own masks; these are not provided by the facility. Discarding used masks in the facility is prohibited.
Safety goggles:to protect eyesall users are strongly encouraged bringing and wearing their own safety goggle if they use oculars. This is optional for glass wearers. If no goggles/glasses are available, one must use cling foil provided by the facility to wrap the whole ocular.
Gloves: users can wear gloves only for sample handling. Gloves must be avoided on any common contact points.
Keep min. 2 meter distanceto any other person! Luckily, the facility has two entrances. Use the door, which is closer to the microscope, you wish to work with! The main room is spacious; for sample preparation and storage use the bench, that is closer to your microscope.
No visitors and no students to watch!One must not be accompaniedby any other person when entering the facility and/or while doing microscopy.
In case of coughing/sneezing (in elbow), disinfect the potentially contaminated area and equipment immediately.
You must not enter the facilitywhen having fever or you show any known symptom of SARS-CoV-2 infection.
First check on the internet calendar, how many microscopes (including the Imaris workstation) have been booked for your time slot. In the main room, max. two people can work at the same time. One additional user can work in the smaller cabinet. Usage of the laser microdissection microscope is allowed upon previous consulting with the head of the facility. Please note, that you must check the availability of other microscopes to be sure about the number of the users working in the same room at the same time!
It is forbidden to walk in to see if a microscope is free! All the checking and booking must be done online. Make sure to finish your experiment within the duration of your booking, including data saving and disinfection.
A 15 minutes break between individual bookings is mandatory to allow appropriate air exchange.
ENTERING THE FACILITY
Always wash/disinfect your hands when entering or leaving the rooms of the facility. There are two disinfection stations at the two entries of the facility and another two stations inside the laboratory rooms. One can find soap by the sink and sufficient paper towels.
Do not wear gloves when entering/leaving the room. You can only use gloves for sample preparation.
Before your first session after re-opening, you must read the COVID-19 guidelines and safety rules over and with your signature you approve to keep these rules and work in the facility accordingly.
In addition to sign in the logbook of a microscope, one must sign in the COVID-19 Logbook placed next to the data transfer workstation. You must indicate the date, your name, department, time point of entering and leaving the facility; your signature is also required.
USAGE OF THE MICROSCOPES, WORKSTATIONS
Only 1 person is allowed to do imaging with a given microscope at any time.
Practical courses, demonstrations must not be held till further notice.
Working in the facility on weekends and on national holidays is not allowed any more.
Training is only possible upon previous agreement with the facility head. Please, do not expect to make an appointment in short notice.
Pilot studies will still be possible and shall be organized with the head of the facility.
External users are welcome; they of course have to accept the new safety rules and use the facility equipment accordingly.
Having troubles (hardware or software problems) during your session, please call the facility head (01-47654 99310).
Equipment contact points will be coated with cling foil. Wipe these areas with 70% EtOH before and after your session! Alternatively, exchange the wrap before you start your experiment.
Equipment contact points include: Keyboards, joysticks, mouse control panels and touch-screens of microscopes, focus knobs/focus drives, condenser arm (inverted microscope), manual switch for TL detector (upright confocal microscopes), housing of the ocular manual stage drivers, incubator door handles (LiveCell mic.)
When doing microscopy try to minimize the number of contact points (ex. do not use focus buttons on the stand, use the joystick and touch screen instead)! If possible, control the microscope functions via the software.
Evidently, computer tables, bench surfaces must be disinfected, too!
Microscope stations will be marked by curtains. Do not draw these curtains, their position allows spatial separation as well as proper air flow.
It is forbidden to use computer tables for sample preparation or storage!
Only use the 70% EtOH provided!Never use any home-made solutions or WHO-disinfectant.
Never remove any disinfectant!
Only contact points of microscopes, computers must be disinfected. Do not try to clean the whole microscope.
Disinfect all contact points when starting and upon completion of your experiment. Disinfection means thorough cleaning of all used surfaces with paper towel wetted by 70% EtOH. You must not spray EtOH directly on to keyboards, joysticks, computer mouse, monitors or any parts of a microsocpe. Only bench surfaces can be directly sprayed.
One must not use paper towels to clean the optics (any lens) of a microscope! Only lens papers can be used.
Although safety materials (disinfectant, paper towel, EtOH, lens paper, foil) are checked daily, in case a stock runs empty, please, call 01-47654 99310 or send an email to imagingcenter(at)boku.ac.at
Please, use your own mobile for calling. In case you use one of telephones in the laboratory, disinfect its wrapping or, alternatively, change it.
Although safety goes first, try to economize the consumption of provided materials.