Arbeits- und Forschungsfeld

Das Interesse an der biologischen Funktion der Protein-Glykosylierung – eines Phänomens, welches fast alle Proteine im Serum und auf Zelloberflächen betrifft – bedingt das Vorhandensein einer leistungsfähigen Analytik in Form der Flüssigchromatographie-Massenspektometrie (LC-MS). So diese nun erfreulicherweise zur Verfügung steht, kann sie für vielfältigste Aufgaben der Protein- sowie eben auch Glykoprotein-Analyse eingesetzt werden.

Zu den Forschungsbereichen

Aktuelle Themen für Diplomarbeiten (aka Masterarbeiten)

Beispiele für mögliche Themen:

-  Herstellung neuartiger Glyco-Vakzine
-  Isomer-spezifische massenspektroskopische Glykan Analytik
-  Aufklärung der Struktur der neuartigen N-Glykane essbarer Algen
-  Glykoproteine in der Allergiediagnostik

Anfragen werden gerne beantwortet

Ausgewählte Arbeiten aus der AG Altmann

Helm J, Grünwald-Gruber C, Thader A, Urteil J, Führer J, Stenitzer D, Maresch D, Neumann L, Pabst M, Altmann F. (2021) Bisecting Lewis X in Hybrid-Type N-Glycans of Human Brain Revealed by Deep Structural Glycomics. Anal Chem. 93, 15175-15182

Mócsai R, Blaukopf M, Svehla E, Kosma P, Altmann F. (2020) The N-glycans of Chlorella sorokiniana and a related strain contain arabinose but have strikingly different structures. Glycobiology 30, 663-676.

Mócsai R, Figl R, Sützl L, Fluch S, Altmann F. (2020) A first view on the unsuspected intra-genus diversity of N-glycans in Chlorella microalgae. Plant J. 103, 184-196.

Mócsai R, Figl R, Troschl C, Strasser R, Svehla E, Windwarder M, Thader A, Altmann F. (2018) N-glycans of the microalga Chlorella vulgaris are of the oligomannosidic type but highly methylated.  Sci Rep. 9(1):331. doi: 10.1038/s41598-018-36884-1.

Grünwald-Gruber C, Thader A, Maresch D, Dalik T, Altmann F (2017) Determination of true ratios of different N-glycan structures in electrospray ionization-mass spectrometry. Anal. Bioanal. Chem . 409(10):2519-2530   doi: 10.1007/s00216-017-0235-8.

Maresch D, Altmann F. (2016) Isotype-specific glycosylation analysis of mouse immunoglobulin G by liquid chromatography-mass spectrometry. Proteomics. 16, 1321-1330.  doi: 10.1002/pmic.201500367.

Windwarder M, Figl R, Svehla E, Mócsai RT, Farcet JB, Staudacher E, Kosma P, Altmann F. (2016) "Hypermethylation" of anthranilic acid-labeled sugars confers the selectivity required for liquid chromatography-mass spectrometry. Anal Biochem 514, 24-31   doi: 10.1016/j.ab.2016.09.008

Windwarder M, Altmann F. (2014) Site-specific analysis of the O-glycosylation of bovine fetuin by electron-transfer dissociation mass spectrometry. J Proteomics 108,  258-268

Holzweber F., Svehla E., Fellner W., Dalik T., Stubler S.,  Hemmer W., Altmann F. (2013) Inhibition of IgE binding to cross-reactive carbohydrate determinants enhances diagnostic selectivity. Allergy 68, 1269-1277.

Pabst M., Chang M., Stadlmann J., Altmann F. (2012) Glycan profiles of the 27 N-glycosylation sites of the HIV envelope protein CN54gp140. Biol. Chem. 393, 719–730

Pabst M.,  Grass J., Tögel S., Liebminger E., Strasser R., Altmann F. (2012)  Isomeric analysis of oligomannosidic N-glycans and their dolichol-linked precursors. Glycobiology, 22, 389-399

Grass J., Pabst M., Chang M., Wozny M., Altmann F. (2011) Analysis of recombinant human follicle-stimulating hormone (FSH) by mass spectrometric approaches. Anal. Bioanal. Chem. 400, 2427-2438

Pabst M., Grass J., Fischl R., Léonard R., Jin C., Hinterkörner G., Borth N., Altmann F. (2010) Nucleotide and Sugar Nucleotide Analysis by LC-ESI-MS on Surface-Conditioned Porous Graphitic Carbon. Anal. Chem. 82, 9782-9788.

Stadlmann J, Pabst M, Altmann F. (2010) Analytical and Functional Aspects of Antibody Sialylation.  J. Clin. Immunol. 30, S15-S19.

Stadlmann J., Weber A., Pabst M., Anderle H., Kunert R., Ehrlich H., Schwarz Hans P., Altmann F. (2009) A close look at human immunoglobulin G sialylation and subclass distribution after lectin fractionation. Proteomics 9, 4143-4153

Jin C., Hantusch G., Hemmer W., Stadlmann J., Altmann F. (2008) Affinity of IgE and IgG against cross-reactive carbohydrate determinants on plant and insect glycoproteins. J. Allergy Clin. Immunol. 121, 185-190

Stadlmann J., Pabst M., Kolarich D., Kunert R., Altmann F., (2008) Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharides. Proteomics 8, 2858-2871

Leonard R., Martin P., Bondili J.S., Chambat G., Veit C., Strasser R., Altmann F. (2008) Identification of the Arabidopsis gene encoding the GH95 α1,2-fucosidase active on xyloglucans. Phytochemistry 69, 1983-1988

Pabst, M., Altmann F. (2008) Influence of electrosorption, solvent, temperature and ion polarity on the performance of LC-ESI-MS using graphitic carbon for acidic oligosaccharides. Anal. Chem. 80, 7534-7542

Altmann F. (2007) The role of protein-glycosylation in allergy. Int. Arch. Allergy Immunol. 142, 99-115

Pabst M., Bondili J.S., Stadlmann J., Mach L., Altmann F. (2007) Mass + retention time = structure: A strategy for the analysis of N-glycans by carbon LC-ESI-MS and its application to fibrin N-glycans. Anal. Chem. 79, 5051-5057

Jin C., Bencúrová B., Borth N., Ferko B., Jensen- Jarolim E., Altmann F., Hantusch B. (2006)  Immunoglobulin G specifically binding plant N-glycans with high affinity could be generated in rabbits but not in mice. Glycobiology 16, 349-357

Léonard D., Rendić D., Rabouille C., Wilson I.B.H., Préat H., Altmann F. (2006) The Drosophila fused lobes gene encodes an N-acetylglucosaminidase involved in N-glycan processing. J. Biol. Chem. 281, 4867-4875

Zeleny R., Léonard R., Dorfner G., Dalik T., Kolarich D., Altmann F. (2006) Molecular cloning and characterisation of a plant α1,3/4-fucosidase based on sequence tags from almond fucosidase I. Phytochemistry 67, 641-648

Leonard R., Petersen B.O., Himly M., Kaar W., Wopfner N., Kolarich D., van Ree R., Ebner C., Duus J.,  Ferreira F., Altmann F.  (2005) Two novel types of O-glycans on the mugwort pollen allergen Art v 1 and their role in antibody binding. J. Biol. Chem. 280, 7932-7940

Bencurova M., Hemmer, W., Focke-Tejkl M., Wilson I.B.H., Altmann F. (2004) Specificity of IgG and IgE antibodies against plant and insect glycoprotein glycans determined with artificial glycoforms of human transferrin. Glycobiology 14, 457-466.  

Wilson I.B.H., Zeleny R., Kolarich D., Staudacher E., Stroop C.J.M., Kamerling J.P. and Altmann F. (2001) Analysis of Asn-linked glycans from vegetable foodstuffs:  Widespread occurrence  of Lewis a, core a1,3‑linked fucose and xylose substitutions. Glycobiology 11, 261-274

Leiter H., Mucha I., Staudacher E., Grimm R., Glössl J., Altmann F. (1999) Purification, cDNA cloning and expression of GDP-l-Fuc : Asn-linked GlcNAc a1,3-fucosyltransferase from mung beans. J. Biol. Chem. 274, 21830-21839

Altmann F., Schwihla H., Staudacher E., Glössl J., März L. (1995) Insect cells contain an unusual, membrane-bound beta-N-acetylglucosaminidase probably involved in the processing of protein N-glycans. J. Biol. Chem. 270, 17344-17349

Kubelka V., Altmann F., Staudacher E., Tretter V., März L., Hård K., Kamerling J.P., Vliegenthart J.F.G. (1993) Primary structures of the N-linked carbohydrate chains from honeybee venom phospholipase A2. Eur. J. Biochem. 213, 1193-1204

Tretter V., Altmann F., Kubelka V., März L., Becker W.M. (1993) Fucose linked 1-3 to the core-region of glycoprotein N-glycans creates an important epitope for IgE from honeybee venom allergic individuals. Int. Arch. Allergy Immunol. 102, 259-266 103      

Tretter V., Altmann F., März L. (1991) Peptide-N4-( N-acetyl-beta-glucosaminyl asparagine amidase F cannot release glycans with fucose attached alpha 1->3 to the asparagine-linked N-acetyl-glucosamine residue.  Eur. J. Biochem. 199, 647-652