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Research project (§ 26 & § 27)
Duration : 2019-10-01 - 2020-09-30

3 test series are to be carried out as follows: - Long-term 3D cell culture with mesenchymal stem cells The cells are embedded in the hydrogels and cultivated for 3-5 days. Then they are separated and transferred into a new hydrogel. After passing through, cell count and cell viability are determined. The cells are passed at least three times. From this the cumulative doubling is determined as a function of time and passengers. A statistical group comparison is made. - Isolation of mesenchymal stem cells from umbilical cord and fat tissue in PL matrix The aim is to test the effect of the strength of the carrier on the migration of mesenchymal stem cells from tissue to the carrier (matrix). The migration is checked by high-frequency microscopy. When migration is observed, the cells are harvested and counted. Migration rate and cell counts are compared. - Encapsulation of mesenchymal stem cells for bioreactor cultivation This experiment will test whether a high-strength PL matrix is stable enough to be used in a stirred tank bioreactor. If this is the case, mesenchymal stem cells are encapsulated in beads and cultured for five days in the stirred tank bioreactor at 200 rpm. If the hydrogels are not strong enough, the strength can be increased or the stirring speed reduced. It is checked whether the cells retain their metabolic activity.
Research project (§ 26 & § 27)
Duration : 2019-07-01 - 2020-06-30

Mesenchymal stem cells (MSCs) are important candidates for cell-based therapies in regenerative medicine. MSCs are isolated from donor tissue and then propagated in vitro on 2D plastic surfaces. These traditional culture formats do not match the physiological in vivo situation. MSCs cultured under 3D conditions (e.g., as aggregates) show much higher therapeutic potential. Within the framework of this project, a bioprocess is being developed which enables the isolation and propagation of MSCs continuously under three-dimensional conditions. For this, donor tissue is embedded in a hydrogel, whereupon MSCs migrate from the tissue into the hydrogel. These cells are then propagated as 3D aggregates in a stirred tank bioreactor. This process avoids repetitive passenger traffic, which among other things leads to damage to the surface markers and unwanted genetic changes.
Research project (§ 26 & § 27)
Duration : 2019-04-01 - 2020-09-30

One of the main challenges of the next century is the CO2 emission reduction in a sustainable way in order to mitigate climate change. Further, the increasing food demand of the growing world population has to be covered under preservation of the diversity of natural ecosystems. Our aim is to tap CO2 as a sustainable resource in order to provide a commercial solution for the climate issue. With a novel yeast strain, CO2 can be converted into high-value biomass which is used as an animal feed additive, without using agricultural area.

Supervised Theses and Dissertations