Papers by Gordana Wozniak-Knopp and Florian Rüker (Link to Pubmed)

Selected publications

Introduction to Antibody Engineering
Editors Florian Rüker and Gordana Wozniak-Knopp
Springer, 2020
Part of the Learning Materials in Biosciences book series (LMB)


Sádio F, Stadlmayr G, Eibensteiner K, Stadlbauer K, Rüker F, Wozniak-Knopp G. Methods for Construction of Yeast Display Libraries of Four-Domain T-Cell Receptors. Methods Mol Biol. 2020;2070:223-248. doi:10.1007/978-1-4939-9853-1_13. PubMed PMID: 31625099.

Dietrich S, Gross AW, Becker S, Hock B, Stadlmayr G, Rüker F, Wozniak-Knopp G. Constant domain-exchanged Fab enables specific light chain pairing in heterodimeric bispecific SEED-antibodies. Biochim Biophys Acta Proteins Proteom. 2020 Jan;1868(1):140250. doi: 10.1016/j.bbapap.2019.07.003. Epub 2019 Jul 8. PubMed PMID: 31295556.

Sádio F, Stadlmayr G, Stadlbauer K, Rüker F, Wozniak-Knopp G. Yeast Surface Display and Cell Sorting of Antigen-Binding Fc Fragments. Methods Mol Biol. 2019;1923:287-308. doi: 10.1007/978-1-4939-9024-5_13. PubMed PMID: 30737746.

Vogt S, Stadlmayr G, Stadlbauer K, Sádio F, Andorfer P, Grillari J, Rüker F, Wozniak-Knopp G. Stabilization of the CD81 Large Extracellular Loop with De Novo Disulfide Bonds Improves Its Amenability for Peptide Grafting. Pharmaceutics. 2018 Aug 27;10(3). pii: E138. doi: 10.3390/pharmaceutics10030138. PubMed PMID: 30150531; PubMed Central PMCID: PMC6160918.

Wozniak-Knopp G, Stadlmayr G, Perthold JW, Stadlbauer K, Gotsmy M, Becker S, Rüker F. An antibody with Fab-constant domains exchanged for a pair of CH3 domains. PLoS One. 2018 Apr 9;13(4):e0195442. doi: 10.1371/journal.pone.0195442. eCollection 2018. PubMed PMID: 29630643; PubMed Central PMCID: PMC5891013.

Wozniak-Knopp G, Stadlmayr G, Perthold JW, Stadlbauer K, Woisetschläger M, Sun H, Rüker F. Designing Fcabs: well-expressed and stable high affinity antigen-binding Fc fragments. Protein Eng Des Sel. 2017 Sep 1;30(9):657-671. doi: 10.1093/protein/gzx042. PubMed PMID: 28981753.

Woisetschläger M, Antes B, Borrowdale R, Wiederkum S, Kainer M, Steinkellner H, Wozniak-Knopp G, Moulder K, Rüker F, Mudde GC. In vivo and in vitro activity of an immunoglobulin Fc fragment (Fcab) with engineered Her-2/neu binding sites. Biotechnol J. 2014 Jun;9(6):844-51. doi: 10.1002/biot.201300387. Epub 2014 May 8. PubMed PMID: 24806546.

Wozniak-Knopp G, Bartl S, Bauer A, Mostageer M, Woisetschläger M, Antes B, Ettl K, Kainer M, Weberhofer G, Wiederkum S, Himmler G, Mudde GC, Rüker F. Introducing antigen-binding sites in structural loops of immunoglobulin constant domains: Fc fragments with engineered HER2/neu-binding sites and antibody properties. Protein Eng Des Sel. 2010 Apr;23(4):289-97. doi: 10.1093/protein/gzq005. Epub 2010 Feb 11. PubMed PMID: 20150180.

Wozniak-Knopp G, Rüker F. A C-terminal interdomain disulfide bond significantly stabilizes the Fc fragment of IgG. Arch Biochem Biophys. 2012 Oct 15;526(2):181-7. doi: 10.1016/ Epub 2012 Mar 29. PubMed PMID: 22483683.

Wozniak-Knopp G, Stadlmann J, Rüker F. Stabilisation of the Fc fragment of human IgG1 by engineered intradomain disulfide bonds. PLoS One. 2012;7(1):e30083. doi: 10.1371/journal.pone.0030083. Epub 2012 Jan 17. PubMed PMID: 22272277; PubMed Central PMCID: PMC3260182.