Latest SCI publications

Latest Projects

Research project (§ 26 & § 27)
Duration : 2021-10-01 - 2024-09-30

Genotype determination is very important in forestry as part of management, controlled seed production and the monitoring of the provenance principle. With the upcoming of the second and third generation sequencing techniques (NGS), sequence based approaches are available for routine screening. Especially useful is a method that bridges traditional and new possibilities, with high throughput and statistical power, but easy to use and to implement. The main problem to use sequence information for genotyping, is the bioinformatic processing. One problem is the difficulty to unambiguous determine alleles in heterozygote individuals . A number of pipelines and suggestions had been published, however, most are elaborate or not able to use the whole sequences of a marker but rather single SNPs or length information. The project aims to introduce genotype by amplicon sequencing for standardized genotyping in forestry (SSR-GBAS). Scripts we developed in earlier work will from the base for software solutions with wide applicability. A database for web based collection of alleles called by whole sequences developed and clearly reproduced genotypes on Oak and Pine on up to 200 markers each as proof of concept will be provided. The project will also improve laboratory methods by developing optimized multiplex approaches and the inclusion of candidate genes as well as neutral markers. All together the approach allows a flexible genotyping of forest genetic resources.
Research project (§ 26 & § 27)
Duration : 2020-11-01 - 2022-07-31

The project focuses on the development of suitable measures for the promotion of wild bees and the optimization of habitats using the example of the Vienna Danube Island. The measures will be implemented together with the City of Vienna (MA 45) and their effectiveness will be evaluated. At the same time, wild bee surveys from 2005 will be repeated on selected sites in order to make a shift in the species spectrum of wild bees visible in the longer term.
Research project (§ 26 & § 27)
Duration : 2021-01-01 - 2022-03-31

DNA barcoding or more generally, using DNA sequences for identification of species might represent a promising tool. Therefore, respective reference data bases are established all over the world. Here, I will test whether tropical cave crickets (Rhaphidophoridae) from Southeast Asia could be determined using this technique by answering the following questions: Which proportion of samples could be identified based on yet existing data bases, including the BOLD system? How many genospecies could be separated based on molecular DNA data irrespectively of the exact species identification? Which basic and advanced needs for successful application of DNA barcoding as determination tool could be derived from the present results?

Supervised Theses and Dissertations