791325 Flow cytometry and cell sorting in biotechnology


Type
Lecture
Semester hours
2
Lecturer (assistant)
Borth, Nicole , Schosserer, Markus
Organisation
Offered in
Sommersemester 2019
Languages of instruction
Englisch

Content

-) Flow Cytometry and Cell Sorting: how does it work

-) Safety in the Flow Cytometry Lab

-) Analysis of Production Cell Lines

-) Analysis of Bacterial and Yeast cultures

-) Sorting for high production rates and improved cell performance

-) Cell growth, proliferation and cell death

-) Chromosomes (Flow Karyotyping. Flow-FISH), pH, ROS, Ca, bead arrays, rare events

-) Experimental design and data evaluation: practical examples

Previous knowledge expected

Basic knowledge of cell biology strongly recommended! This course is recommended for master and PhD-students.

Objective (expected results of study and acquired competences)

Students will know the basic principles and applications of flow cytometry and cell sorting and their use in biotechnology. Participants will be able to identify important aspects of flow cytometric analyses and controls and will be able to design and implement experiments and to interpret results.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.