791327 Practical course in plant biotechnology (in Eng.)


Art
Übung
Semesterstunden
3
Vortragende/r (Mitwirkende/r)
Laimer, Margit
Organisation
Angeboten im Semester
Sommersemester 2019
Unterrichts-/ Lehrsprachen
Englisch

Lehrinhalt

Preparation of culture media, working under aseptic conditions; surface sterilization and preparation of the source isolate (tissue); initiation and passaging of callus cultures and suspension cultures, explants; isolation of protoplasts; exercises to study the influence of periphysis, cyclophysis and topophysis on the establishment of cultures, mediated transformation by means of Agrobacterium, GUS assay

Inhaltliche Voraussetzungen (erwartete Kenntnisse)

791111 Plant Biotechnology

Lehrziel

Students will be experienced in the practical implementation of the techniques of in-vitro culture and protein analyses:
+ know how to establish, maintain, propagate different samples of plant tissues in vitro
+ handle the transfer to greenhouse conditions
+ be able to induce somatic embryogenesis
+ carry out transformation experiments
+ verify the transgenic status of plant material
Noch mehr Informationen zur Lehrveranstaltung, wie Termine oder Informationen zu Prüfungen, usw. finden Sie auf der Lehrveranstaltungsseite in BOKUonline.