790414 Instructional course IIIB - applied virology (in Eng.)


Art
Übung
Semesterstunden
2
Vortragende/r (Mitwirkende/r)
Klausberger, Miriam , Laimer, Margit
Organisation
Angeboten im Semester
Wintersemester 2020/21
Unterrichts-/ Lehrsprachen
Englisch

Lehrinhalt

One part gives insight into the methodology of insect cell culture and the baculovirus expression system. Insect cells will be transfected with recombinant baculovirus DNA, the viral titer will be determined by plaque-assay and TCID50 and expression of a fluorescence reporter protein will be optimized using different insect cell infection protocols.
The other part will deal with plant viruses including mechanical inoculation of indicator plants and ELISA testing for high throughput detection of plant viruses.

Lehrziel

After completion of this course students can outline virological methods in the fields of insect cell culture as well as in plant virology and are able to carry out transfection/infection of cells, perform virus titrations, mechanical inoculation of plants and ELISA tests and to visualize and characterize infected cells using fluorescence microscopy.
Noch mehr Informationen zur Lehrveranstaltung, wie Termine oder Informationen zu Prüfungen, usw. finden Sie auf der Lehrveranstaltungsseite in BOKUonline.