790397 Protein engineering (in Eng.)


Art
Vorlesung
Semesterstunden
3
Vortragende/r (Mitwirkende/r)
Rüker, Florian , Wozniak-Knopp, Gordana , Peterbauer, Clemens Karl
Organisation
Angeboten im Semester
Wintersemester 2021/22
Unterrichts-/ Lehrsprachen
Englisch

Lehrinhalt

These is the approximate schedule:

1GWK FR Antibodies
2GWK FR Antibodies
3GWK Design of alternative binding scaffolds
4CP Enzyme evolution
5CP Enzyme evolution
6FR Top 50 Drugs
7GWK CP Technology of library construction
8GWK Difficult-to-express proteins
9FR Covid19 vaccines in development
10CP High throughput screening / microfluidics
11GWK Fusion proteins
12GWK CP FR Wrap up and first exam



•Antibodies
•Vaccines
•Influenza / HIV / HPV
•Difficult-to-express proteins
•Fusion proteins
•Covid19 vaccines in development
•High throughput screening / microfluidics
•Enzyme evolution
•Technology of library construction
•Design of alternative binding scaffolds

Inhaltliche Voraussetzungen (erwartete Kenntnisse)

Basic knowledge in molecular biology and biochemistry
Protein structure and function

Lehrziel

Students who successfully complete this course will be able to:
Lecture 1: Antibodies
Learning objectives:
-To describe the antibody fold, antibody domain structure and molecular architecture
-To define the beneficial biological properties of antibodies and relate them to molecular structure
-To comment on the importance of antibody-based therapeutics in present times

Lectures 2 and 3: anti-viral Vaccines
Learning objectives:
-To describe various approaches how protein engineering helps to design better vaccines
-To introduce rational, computational, and combinatorial strategies for vaccine design
-To define the requirements for successful vaccines and how these can be met by protein engineering approaches
-To compare and discuss the selected examples for vaccines that are based on protein engineering

Lecture 4: Difficult-to-express proteins
Learning objectives:
-To describe the properties of Difficult-to-express proteins as well as their role as therapeutic targets
-To evaluate various approaches and methods to improve solubility, folding, enable crystallization and prevent aggregation and formation of inclusion bodies

Lecture 5: Fusion proteins
Learning objectives:
-To comment on the advantages of fusion proteins intended for therapeutic purposes, bioanalytics and industrial applications
-To compare different designs of fusion proteins and corresponding tags
-To discuss challenges in expression, production and analytics of fusion proteins

Lecture 6: Covid19 vaccines in development
Learning objectives:
-To describe which types of vaccines against Covid19 are currently in development
-To discuss the different strategies and approaches

Lecture 7: High throughput screening / microfluidics
Learning objectives:
- To understand the principal characteristics of enzyme activity assays: localization of enzyme, nature of substrate and product, detectable and quantifyable molecules
- Knowledge of factors that influence the detection of a product/byproduct/secondary product of an enzymatic reaction and its link to the genotype

Lecture 8: Enzyme evolution
Learning objectives:
- To understand the drivers of evolution of enzyme activity: mutation and recombination, selective pressure, trade-offs between stability and dynamics
- To describe the acquisition of novel functions and reactivities

Lecture 9 and 10: Technology of library construction
Learning objectives:
-To describe the concept of library display and selection
-To compare various library display systems
-To choose a proper mutagenesis method for the intended protein modification
-To design a selection process protocol for the desired change of protein function

Lecture 11: Design of alternative binding scaffolds
Learning objectives:
-To identify the key properties of a functional binding scaffold
-To implement the knowledge on protein structure in designing a binding surface
-To apply the principles of library selection methods to the design of a binding scaffold
Noch mehr Informationen zur Lehrveranstaltung, wie Termine oder Informationen zu Prüfungen, usw. finden Sie auf der Lehrveranstaltungsseite in BOKUonline.