752346 Applied biocatalysis (in Eng.)

Art
Vorlesung
Semesterstunden
3
Vortragende/r (Mitwirkende/r)
Organisation
Angeboten im Semester
Sommersemester 2023
Unterrichts-/ Lehrsprachen
Englisch
Lehrinhalt

"Applied biocatalysis" is an advanced lecture within the specialisation of “Protein engineering and technology”; in the master study programme Biotechnology H418. This course is based on the knowledge and concepts provided in engineering, chemistry, biochemistry, and molecular biology courses. The lecture starts with a general overview on enzymatic reactions, screening and engineering of enzymes for industrial applications, followed by the basics of biochemical reaction engineering (reaction and process engineering) and general criteria for industrial enzymatic processes. In the main part of the lecture specific examples of biocatalytic synthetic reactions of oxidoreductases, transferases, hydrolases, lyases, isomerases, and ligases in the various industrial sectors are introduced, analysed and probable improvements are discussed. Finally, general rules to establish a biocatalytic process will be the drawn and the strengths and weaknesses of biocatalytic reactions will be assessed.
Inhaltliche Voraussetzungen (erwartete Kenntnisse)

Knowledge of enzymatic reactions, engineering and (bio-)chemistry.
Lehrziel

After successful attendance of this lecture the participants can recall the application of enzymes for the synthesis of bulk chemicals, fine chemicals and specialities. They can differentiate between different biocatalysts and know their cosubstrate requirements and necessary auxiliary reactions. They can evaluate the usefulness and performance of equilibrium reactions, enantiomeric reactions, hydrolytic reactions, reductive reactions, oxidation reactions, oxygenation reactions, peroxidation reactions, Bayer-Villiger reactions, formation of C-C bonds, and de-/halogenation reactions. They can identify critical mechanistic limitations of reactions and rate limiting steps, and are competent to optimise reaction conditions. Finally, participants will be able to plan biocatalytic processes based on existing industrial processes and calculate performance numbers for the comparison of processes.
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