Scientific article: DNA polyelectrolyte multilayer coatings are antifouling and promote mammalian cell adhesion

DNA polyelectrolyte multilayer coatings impaired biofilm formation of Staphylococcus while supporting osteoblast-like cell attachment. Thus these coatings could reduce infection risk while promoting host tissue cell attachment on implants in orthopedic and dental applications.


Implant-associated infections are generally difficult to treat as biofilm growth protects microorganisms from the host´s immune response and antibiotics. Therefore, modifications of implant surfaces that can prevent or reduce bacterial adhesion and biofilm formation while supporting tissue cell attachment and spreading are desired for orthopedic and dental applications.

We developed polyelectrolyte multilayer coatings using chitosan and DNA on implant materials (PMMA and titanium) via the layer-by-layer technique. The DNA multilayer coatings impaired biofilm formation of Staphylococcus by ~90% on both PMMA and titanium surfaces. In addition, coatings demonstrated no cytotoxic effect on osteoblast-like (SaOS-2) cells.

You can read more about our novel findings in Materials 2021, 14(6), 4596.