Nanostructured scaffolds accelerate differentiation of neural stem cells


In the work, "Nanostructured scaffolds based on bioresorbable polymers and graphene oxide induce the aligned migration and accelerate the neuronal differentiation of neural stem cells", the fabrication and characterization of biodegradable nanostructured poly(L-lactide-co-ε-caprolactone) (PLCL) is described. These scaffolds, either with or without graphene oxide (GO) functionalization, support the alignment, growth, migration and differentiation of neural stem and progenitor cells without a need of a laminin coating.

FDr Jagoba Iturri and Professor José Luis Toca-Herrera from the Institute for Biophysics (Department of Nanobiotechnology) have contributed by means of Atomic Force Microscopy to characterize the topographical features of these layered systems as well as to identify the resulting forces from successful application of the functional coatings.

Within the field of neural tissue engineering, there is a huge need for the development of materials that promote the adhesion, aligned migration and differentiation of stem cells into neuronal and supportive glial cells. In this study, we have fabricated bioresorbable elastomeric scaffolds combining an ordered nanopatterned topography together with a surface functionalization with graphene oxide (GO) in mild conditions. These scaffolds allowed the attachment of murine neural stem cells (NSCs) without the need of any further coating of its surface with extracellular matrix adhesion proteins. The NSCs were able to give rise to both immature neurons and supporting glial cells over the nanostructured scaffolds in vitro, promoting their aligned migration in cell clusters following the nanostructured grooves. This system has the potential to reestablish spatially oriented neural precursor cell connectivity, constituting a promising tool for future cellular therapy including nerve tissue regeneration.

Read more in: Nanostructured scaffolds based on bioresorbable polymers and graphene oxide induce the aligned migration and accelerate the neuronal differentiation of neural stem cells, Nanomedicine: Nanotechnology, Biology and Medicine, 12. October 2020, https://doi.org/10.1016/j.nano.2020.102314


10.11.2020