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Further information can be found at:

 

 

 

118. Kendlbacher, F. L.; Bloch, S.; Hager-Mair, F. F.; Bacher, J.; Janesch, B.; Thurnheer, T.; Andrukhov, O.; Schäffer, C., Multispecies biofilm behavior and host interaction support the association of Tannerella serpentiformis with periodontal health. Mol Oral Microbiol 2022. doi: 10.1111/omi.12385.

117. Legg, M. S. G.; Hager-Mair, F. F.; Krauter, S.; Gagnon, S. M. L.; Lòpez-Guzmán, A.; Lim, C.; Blaukopf, M.; Kosma, P.; Schäffer, C.; Evans, S. V., The S-layer homology domains of Paenibacillus alvei surface protein SpaA bind to cell wall polysaccharide through the terminal monosaccharide residue. J Biol Chem 2022, 101745. doi: 10.1016/j.jbc.2022.101745.

116. Braun, M. L.; Tomek, M. B.; Grünwald-Gruber, C.; Nguyen, P. Q.; Bloch, S.; Potempa, J. S.; Andrukhov, O.; Schäffer, C., Shut-down of type IX protein secretion alters the host immune response to Tannerella forsythia and Porphyromonas gingivalis. Front Cell Infect Microbiol 2022, 12, 835509. doi: 10.3389/fcimb.2022.835509.

115. Tomek, M. B.; Janesch, B.; Braun, M. L.; Taschner, M.; Figl, R.; Grünwald-Gruber, C.; Coyne, M. J.; Blaukopf, M.; Altmann, F.; Kosma, P.; Kählig, H.; Comstock, L. E.; Schäffer, C., A combination of structural, genetic, phenotypic and enzymatic analyses reveals the importance of a predicted fucosyltransferase to protein O-glycosylation in the Bacteroidetes. Biomolecules 2021, 11, (12). doi: 10.3390/biom11121795.

114. Hager-Mair, F. F.; Stefanović, C.; Lim, C.; Webhofer, K.; Krauter, S.; Blaukopf, M.; Ludwig, R.; Kosma, P.; Schäffer, C., Assaying Paenibacillus alvei CsaB-catalysed ketalpyruvyltransfer to saccharides by measurement of phosphate release. Biomolecules 2021, 11, (11). doi: 10.3390/biom11111732.

113. Pultar, F.; Hansen, M. E.; Wolfrum, S.; Böselt, L.; Fróis-Martins, R.; Bloch, S.; Kravina, A. G.; Pehlivanoglu, D.; Schäffer, C.; LeibundGut-Landmann, S.; Riniker, S.; Carreira, E. M., Mutanobactin D from the human microbiome: Total synthesis, configurational assignment, and biological evaluation. J Am Chem Soc 2021, 143, (27), 10389-10402. doi: 10.1021/jacs.1c04825.

112. Stefanović, C.; Hager, F. F.; Schäffer, C., LytR-CpsA-Psr glycopolymer transferases: Essential bricks in Gram-positive bacterial cell wall assembly. Int J Mol Sci 2021, 22, (2), 908. doi: 10.3390/ijms22020908.

111. Krauter, S.; Schäffer, C.; Kosma, P., Synthesis of a pyruvylated N-acetyl-β-D-mannosamine containing disaccharide repeating unit of a cell wall glycopolymer from Paenibacillus alvei. Arkivoc 2021, 137-151. doi: 10.24820/ark.5550190.p011.358.

110. Hottmann, I.; Borisova, M.; Schäffer, C.; Mayer, C., Peptidoglycan salvage enables the periodontal pathogen Tannerella forsythia to survive within the oral microbial community. Microb Physiol 2021, 1-12. doi: 10.1159/000516751.

109. Cox, E.; Aloulou, M.; Fleckenstein, J. M.; Schäffer, C.; Sjöling, Å.; Schüller, S.; Hanevik, K.; Devriendt, B.; Zhang, W.; Svennerholm, A. M.; Dudley, E. G., The intriguing interaction of Escherichia coli with the host environment and innovative strategies to interfere with colonization: a summary of the 2019 E. coli and the mucosal immune system Meeting. Appl Environ Microbiol 2020, 86, (24). doi: 10.1128/aem.02085-20.

108. Mayer, V. M. T.; Tomek, M. B.; Figl, R.; Borisova, M.; Hottmann, I.; Blaukopf, M.; Altmann, F.; Mayer, C.; Schäffer, C., Utilization of different MurNAc sources by the oral pathogen Tannerella forsythia and role of the inner membrane transporter AmpG. BMC Microbiol 2020, 20, (1), 352. doi: 10.1186/s12866-020-02006-z.

107. Behm, C.; Blufstein, A.; Abhari, S. Y.; Koch, C.; Gahn, J.; Schäffer, C.; Moritz, A.; Rausch-Fan, X.; Andrukhov, O., Response of human mesenchymal stromal cells from periodontal tissue to LPS depends on the purity but not on the LPS source. Mediators Inflamm 2020, 8704896. doi: 10.1155/2020/8704896.

106. Zwickl, N. F.; Stralis-Pavese, N.; Schäffer, C.; Dohm, J. C.; Himmelbauer, H., Comparative genome characterization of the periodontal pathogen Tannerella forsythia. BMC Genomics 2020, 21, (1), 150. doi: 10.1186/s12864-020-6535-y.

105. Hager, F. F.; Sützl, L.; Stefanovic, C.; Blaukopf, M.; Schäffer, C., Pyruvate substitutions on glycoconjugates. Int J Mol Sci 2019, 20, (19), pii: E4929. doi: 10.3390/ijms20194929.

104. Mayer, V. M. T.; Hottmann, I.; Figl, R.; Altmann, F.; Mayer, C.; Schäffer, C., Peptidoglycantype analysis of the N-acetylmuramic acid auxotrophic oral pathogen Tannerella forsythia and reclassification of the peptidoglycan-type of Porphyromonas gingivalis. BMC Microbiol 2019, 19, (1), 200. doi: 10.1186/s12866-019-1575-7.

103. Bloch, S.; Tomek, M. B.; Friedrich, V.; Messner, P.; Schäffer, C., Nonulosonic acids contribute to the pathogenicity of the oral bacterium Tannerella forsythia. Interface Focus 2019, 9, (2), 20180064. doi: 10.1098/rsfs.2018.0064.

102. Megson, Z. A.; Messner, P.; Schäffer, C., Assaying Fucosidase Activity. In Bacterial Polysaccharides: Methods and Protocols, Methods in Molecular Biology, Brockhausen, I., Ed. Humana Press, NY: New York, 2019; Vol. 1954, pp 269-278. doi: 10.1007/978-1-4939-9154- 9_21.

101. Tomek, M. B.; Maresch, D.; Windwarder, M.; Friedrich, V.; Janesch, B.; Fuchs, K.; Neumann, L.; Nimeth, I.; Zwickl, N. F.; Dohm, J. C.; Everest-Dass, A.; Kolarich, D.; Himmelbauer, H.; Altmann, F.; Schäffer, C., A general protein O-glycosylation gene cluster encodes the speciesspecific glycan of the oral pathogen Tannerella forsythia: O-glycan biosynthesis and immunological implications. Front Microbiol 2018, 9, 2008. doi: 10.3389/fmicb.2018.02008.

100. Blackler, R. J.; López-Guzmán, A.; Hager, F. F.; Janesch, B.; Martinz, G.; Gagnon, S. M. L.; HajiGhassemi, O.; Kosma, P.; Messner, P.; Schäffer, C.; Evans, S. V., Structural basis of cell wall anchoring by SLH domains in Paenibacillus alvei. Nat Commun 2018, 9, (1), 3120. doi: 10.1038/s41467-018-05471-3.

99. Hager, F. F.; Lopez-Guzman, A.; Krauter, S.; Blaukopf, M.; Polter, M.; Brockhausen, I.; Kosma, P.; Schäffer, C., Functional characterization of enzymatic steps involved in pyruvylation of bacterial secondary cell wall polymer fragments. Front Microbiol 2018, 9, 1356. doi: 10.3389/fmicb.2018.01356.

98. Schäffer, C.; Messner, P., Carb loading takes proteins on a ride. Journal of Biological Chemistry 2018, 293, (14), 5374-5375. doi: 10.1074/jbc.H118.002411.

97. Rath, C. B.; Schirmeister, F.; Figl, R.; Seeberger, P. H.; Schäffer, C.; Kolarich, D., Flagellin glycoproteomics of the periodontitis associated pathogen Selenomonas sputigena reveals previously not described O-glycans and rhamnose fragment rearrangement occurring on the glycopeptides. Mol Cell Proteomics 2018, 17, (4), 721-736. doi: 10.1074/mcp.RA117.000394.

96. Bloch, S.; Zwicker, S.; Bostanci, N.; Sjöling, A.; Bostrom, E. A.; Belibasakis, G. N.; Schäffer, C., Immune response profiling of primary monocytes and oral keratinocytes to different Tannerella forsythia strains and their cell surface mutants. Mol Oral Microbiol 2018, 33, (2), 155-167. doi: 10.1111/omi.12208.

95. Bönisch, E.; Oh, Y. J.; Anzengruber, J.; Hager, F. F.; Lopez-Guzman, A.; Zayni, S.; Hinterdorfer, P.; Kosma, P.; Messner, P.; Duda, K. A.; Schäffer, C., Lipoteichoic acid mediates binding of a Lactobacillus S-layer protein. Glycobiology 2018, 28, (3), 148-158. doi: 10.1093/glycob/cwx102.

94. Hottmann, I.; Mayer, V. M. T.; Tomek, M. B.; Friedrich, V.; Calvert, M. B.; Titz, A.; Schäffer, C.; Mayer, C., N-Acetylmuramic acid (MurNAc) auxotrophy of the oral pathogen Tannerella forsythia: Characterization of a MurNAc kinase and analysis of its role in cell wall metabolism. Front Microbiol 2018, 9, 19. doi: 10.3389/fmicb.2018.00019.

93. Bloch, S.; Thurnheer, T.; Murakami, Y.; Belibasakis, G. N.; Schäffer, C., Behavior of two Tannerella forsythia strains and their cell surface mutants in multispecies oral biofilms. Mol Oral Microbiol 2017, 32, (5), 404-418. doi: 10.1111/omi.12182.

92. Lane, R. S.; St Ange, K.; Zolghadr, B.; Liu, X.; Schäffer, C.; Linhardt, R. J.; DeAngelis, P. L., Expanding glycosaminoglycan chemical space: towards the creation of sulfated analogs, novel polymers and chimeric constructs. Glycobiology 2017, 27, (7), 646-656. doi: 10.1093/glycob/cwx021.

91. Tomek, M. B.; Janesch, B.; Maresch, D.; Windwarder, M.; Altmann, F.; Messner, P.; Schäffer, C., A pseudaminic acid or a legionaminic acid derivative transferase is strain-specifically implicated in the general protein O-glycosylation system of the periodontal pathogen Tannerella forsythia. Glycobiology 2017, 27, (6), 555-567. doi: 10.1093/glycob/cwx019.

90. Anzengruber, J.; Bublin, M.; Bonisch, E.; Janesch, B.; Tscheppe, A.; Braun, M. L.; Varga, E. M.; Hafner, C.; Breiteneder, H.; Schäffer, C., Lactobacillus buchneri S-layer as carrier for an Ara h 2-derived peptide for peanut allergen-specific immunotherapy. Mol Immunol 2017, 85, 81- 88. doi: 10.1016/j.molimm.2017.02.005.

89. Friedrich, V.; Janesch, B.; Windwarder, M.; Maresch, D.; Braun, M. L.; Megson, Z. A.; Vinogradov, E.; Goneau, M. F.; Sharma, A.; Altmann, F.; Messner, P.; Schoenhofen, I. C.; Schäffer, C., Tannerella forsythia strains display different cell-surface nonulosonic acids: biosynthetic pathway characterization and first insight into biological implications. Glycobiology 2017, 27, (4), 342-357. doi: 10.1093/glycob/cww129.

88. Schäffer, C.; Messner, P., Emerging facets of prokaryotic glycosylation. FEMS Microbiology Reviews 2017, 41, (1), 49-91. doi: 10.1093/femsre/fuw036.

87. Stafford, G. P.; Chaudhuri, R. R.; Haraszthy, V.; Friedrich, V.; Schäffer, C.; Ruscitto, A.; Honma, K.; Sharma, A., Draft genome sequences of three clinical isolates of Tannerella forsythia isolated from subgingival plaque from periodontitis patients in the United States. Genome Announc 2016, 4, (6). doi: 10.1128/genomeA.01286-16.

86. Ruscitto, A.; Hottmann, I.; Stafford, G. P.; Schäffer, C.; Mayer, C.; Sharma, A., Identification of a novel N-acetylmuramic acid transporter in Tannerella forsythia. J Bacteriol 2016, 198, (22), 3119-3125. doi: 10.1128/JB.00473-16.

85. van Teeseling, M. C.; Maresch, D.; Rath, C. B.; Figl, R.; Altmann, F.; Jetten, M. S.; Messner, P.; Schäffer, C.; van Niftrik, L., The S-layer protein of the anammox bacterium Kuenenia stuttgartiensis is heavily O-glycosylated. Front Microbiol 2016, 7, 1721. doi: 10.3389/fmicb.2016.01721.

84. Janesch, B.; Schirmeister, F.; Maresch, D.; Altmann, F.; Messner, P.; Kolarich, D.; Schäffer, C., Flagellin glycosylation in Paenibacillus alvei CCM 2051T. Glycobiology 2016, 26, (1), 74-87. doi: 10.1093/glycob/cwv087.

83. Friedrich, V.; Gruber, C.; Nimeth, I.; Pabinger, S.; Sekot, G.; Posch, G.; Altmann, F.; Messner, P.; Andrukhov, O.; Schäffer, C., Outer membrane vesicles of Tannerella forsythia: biogenesis, composition, and virulence. Molecular Oral Microbiology 2015, 30, (6), 451-473. doi: 10.1111/omi.12104.

82. Megson, Z. A.; Pittenauer, E.; Duda, K. A.; Engel, R.; Ortmayr, K.; Koellensperger, G.; Mach, L.; Allmaier, G.; Holst, O.; Messner, P.; Schäffer, C., Inositol-phosphodihydroceramides in the periodontal pathogen Tannerella forsythia: Structural analysis and incorporation of exogenous myo-inositol. Biochim Biophys Acta 2015, 1851, (11), 1417-1427. doi: 10.1016/j.bbalip.2015.08.004.

81. Friedrich, V.; Pabinger, S.; Chen, T.; Messner, P.; Dewhirst, F. E.; Schäffer, C., Draft genome sequence of Tannerella forsythia type strain ATCC 43037. Genome Announc 2015, 3, (3), e00660-15. doi: 10.1128/genomeA.00660-15.

80. Zolghadr, B.; Gasselhuber, B.; Windwarder, M.; Pabst, M.; Kracher, D.; Kerndl, M.; Zayni, S.; Hofinger-Horvath, A.; Ludwig, R.; Haltrich, D.; Oostenbrink, C.; Obinger, C.; Kosma, P.; Messner, P.; Schäffer, C., UDP-sulfoquinovose formation by Sulfolobus acidocaldarius. Extremophiles 2015, 19, (2), 451-467. doi: 10.1007/s00792-015-0730-9.

79. Megson, Z. A.; Koerdt, A.; Schuster, H.; Ludwig, R.; Janesch, B.; Frey, A.; Naylor, K.; Wilson, I.; Stafford, G. P.; Messner, P.; Schäffer, C., Characterization of an α-L-fucosidase from the periodontal pathogen Tannerella forsythia. Virulence 2015, 6, (3), 282-292. doi: 10.1080/21505594.2015.1010982.

78. Tomek, M. B.; Neumann, L.; Nimeth, I.; Koerdt, A.; Andesner, P.; Messner, P.; Mach, L.; Potempa, J. S.; Schäffer, C., The S-layer proteins of Tannerella forsythia are secreted via a type IX secretion system that is decoupled from protein O-glycosylation. Mol Oral Microbiol 2014, 29, (6), 307-320. doi: 10.1111/omi.12062.

77. Anzengruber, J.; Pabst, M.; Neumann, L.; Sekot, G.; Heinl, S.; Grabherr, R.; Altmann, F.; Messner, P.; Schäffer, C., Protein O-glucosylation in Lactobacillus buchneri. Glycoconj J 2014, 31, (2), 117-131. doi: 10.1007/s10719-013-9505-7.

76. Anzengruber, J.; Courtin, P.; Claes, I. J. J.; Debreczeny, M.; Hofbauer, S.; Obinger, C.; Chapot-Cortier, M.-P.; Vanderleyden, J.; Messner, P.; Schäffer, C., Biochemical characterization of the major N-acetylmuramidase from Lactobacillus buchneri. Microbiology 2014, 160, (8), 1807-1819. doi: 10.1099/mic.0.078162-0.

75. Oh, Y. J.; Sekot, G.; Duman, M.; Chtcheglova, L.; Messner, P.; Peterlik, H.; Schäffer, C.; Hinterdorfer, P., Characterizing the S-layer structure and anti-S-layer antibody recognition on intact Tannerella forsythia cells by scanning probe microscopy and small angle X-ray scattering. J Mol Recognit 2013, 26, (11), 542-549. doi: 10.1002/jmr.2298.

74. Janesch, B.; Koerdt, A.; Messner, P.; Schäffer, C., The S-layer homology domain-containing protein SlhA from Paenibacillus alvei CCM 2051T is important for swarming and biofilm formation. PLoS One 2013, 8, (9), e76566. doi: 10.1371/journal.pone.0076566.

73. Bernardi, A.; Jimenez-Barbero, J.; Casnati, A.; De Castro, C.; Darbre, T.; Fieschi, F.; Finne, J.; Funken, H.; Jaeger, K. E.; Lahmann, M.; Lindhorst, T. K.; Marradi, M.; Messner, P.; Molinaro, A.; Murphy, P. V.; Nativi, C.; Oscarson, S.; Penades, S.; Peri, F.; Pieters, R. J.; Renaudet, O.; Reymond, J. L.; Richichi, B.; Rojo, J.; Sansone, F.; Schaffer, C.; Turnbull, W. B.; VelascoTorrijos, T.; Vidal, S.; Vincent, S.; Wennekes, T.; Zuilhof, H.; Imberty, A., Multivalent glycoconjugates as anti-pathogenic agents. Chem Soc Rev 2013, 42, (11), 4709-27. doi: 10.1039/c2cs35408j.

72. Posch, G.; Andrukhov, O.; Vinogradov, E.; Lindner, B.; Messner, P.; Holst, O.; Schäffer, C., Structure and immunogenicity of the rough-type lipopolysaccharide from the periodontal pathogen Tannerella forsythia. Clin Vaccine Immunol 2013, 20, (6), 945-953. doi: 10.1128/CVI.00139-13.

71. Zamocky, M.; Sekot, G.; Buckova, M.; Godocikova, J.; Schäffer, C.; Farkasovsky, M.; Obinger, C.; Polek, B., Intracellular targeting of ascomycetous catalase-peroxidases (KatG1s). Arch Microbiol 2013, 195, (6), 393-402. doi: 10.1007/s00203-013-0887-5.

70. Sekot, G.; Schuster, D.; Messner, P.; Pum, D.; Peterlik, H.; Schäffer, C., Small-angle X-ray scattering for imaging of surface layers on intact bacteria in the native environment. J Bacteriol 2013, 195, (10), 2408-14. doi: 10.1128/jb.02164-12.

69. Posch, G.; Pabst, M.; Neumann, L.; Coyne, M. J.; Altmann, F.; Messner, P.; Comstock, L. E.; Schäffer, C., "Cross-glycosylation" of proteins in Bacteroidales species. Glycobiology 2013, 23, (5), 568-577. doi: 10.1093/glycob/cws172.

68. Janesch, B.; Messner, P.; Schäffer, C., Are the surface layer homology domains essential for cell surface display and glycosylation of the S-layer protein from Paenibacillus alvei CCM 2051T, J Bacteriol 2013, 195, (3), 565-575. doi: 10.1128/JB.01487-12.

67. Messner, P.; Schäffer, C.; Kosma, P., Bacterial cell-envelope glycoconjugates. Adv Carbohydr Chem Biochem 2013, 69, 209-272. doi: 10.1016/B978-0-12-408093-5.00006-X.

66. Ristl, R.; Janesch, B.; Anzengruber, J.; Forsthuber, A.; Blaha, J.; Messner, P.; Schäffer, C., Description of a putative oligosaccharyl:S-layer protein transferase from the tyrosine O-glycosylation system of Paenibacillus alvei CCM 2051T . Adv Microbiol 2012, 2, (4), 537-546. doi: 10.4236/aim.2012.24069.

65. Posch, G.; Sekot, G.; Friedrich, V.; Megson, Z. A.; Koerdt, A.; Messner, P.; Schäffer, C., Glycobiology aspects of the periodontal pathogen Tannerella forsythia. Biomolecules 2012, 2, (4), 467-482. doi: 10.3390/biom2040467.

64. Ristl, R.; Kainz, B.; Stadlmayr, G.; Schuster, H.; Pum, D.; Messner, P.; Obinger, C.; Schäffer, C., A fusion tag to fold on: the S-layer protein SgsE confers improved folding kinetics to translationally fused enhanced green fluorescent protein. J Microbiol Biotechnol 2012, 22, (9), 1271-8. doi:

63. Sekot, G.; Posch, G.; Oh, Y. J.; Zayni, S.; Mayer, H. F.; Pum, D.; Messner, P.; Hinterdorfer, P.; Schäffer, C., Analysis of the cell surface layer ultrastructure of the oral pathogen Tannerella forsythia. Arch Microbiol 2012, 194, (6), 525-539. doi: 10.1007/s00203-012-0792-3.

62. Poppinga, L.; Janesch, B.; Fünfhaus, A.; Sekot, G.; Garcia-Gonzalez, E.; Hertlein, G.; Hedtke, K.; Schäffer, C.; Genersch, E., Identification and functional analysis of the S-layer protein SplA of Paenibacillus larvae, the causative agent of American Foulbrood of honey bees. PLoS Pathog 2012, 8, (5), e1002716. doi: 10.1371/journal.ppat.1002716.

61. Meyer, B. H.; Zolghadr, B.; Peyfoon, E.; Pabst, M.; Panico, M.; Morris, H. R.; Haslam, S. M.; Messner, P.; Schäffer, C.; Dell, A.; Albers, S.-V., Sulfoquinovose synthase - an important enzyme in the N-glycosylation pathway of Sulfolobus acidocaldarius. Mol Microbiol 2011, 82, (5), 1150-1163. doi: 10.1111/j.1365-2958.2011.07875.x.

60. Posch, G.; Pabst, M.; Brecker, L.; Altmann, F.; Messner, P.; Schäffer, C., Characterization and scope of S-layer protein O-glycosylation in Tannerella forsythia. J Biol Chem 2011, 286, (44), 38714-38724. doi: 10.1074/jbc.M111.284893.

59. Sekot, G.; Posch, G.; Messner, P.; Matejka, M.; Rausch-Fan, X.; Andrukhov, O.; Schäffer, C., Potential of the Tannerella forsythia S-layer to delay the immune response. J Dent Res 2011, 90, (1), 109-114. doi: 10.1177/0022034510384622.

58. Ristl, R.; Steiner, K.; Zarschler, K.; Zayni, S.; Messner, P.; Schäffer, C., The S-layer glycomeadding to the sugar coat of bacteria. Int J Microbiol 2011, (pii: 127870). doi: 10.1155/2011/127870.

57. Zarschler, K.; Janesch, B.; Kainz, B.; Ristl, R.; Messner, P.; Schäffer, C., Cell surface display of chimeric glycoproteins via the S-layer of Paenibacillus alvei. Carbohydr Res 2010, 345, (10), 1422-1431. doi: 10.1016/j.carres.2010.04.010.

56. Zarschler, K.; Janesch, B.; Pabst, M.; Altmann, F.; Messner, P.; Schäffer, C., Protein tyrosine O-glycosylation - a rather unexplored prokaryotic glycosylation system. Glycobiology 2010, 20, (6), 787-798. doi: 10.1093/glycob/cwq035.

55. Steiner, K.; Hagelueken, G.; Messner, P.; Schäffer, C.; Naismith, J. H., Structural basis of substrate binding in WsaF, a rhamnosyltransferase from Geobacillus stearothermophilus. J Mol Biol 2010, 397, (2), 436-47. doi: 10.1016/j.jmb.2010.01.035.

54. Kainz, B.; Steiner, K.; Moller, M.; Pum, D.; Schäffer, C.; Sleytr, U. B.; Toca-Herrera, J. L., Absorption, steady-state fluorescence, fluorescence lifetime, and 2D self-assembly properties of engineered fluorescent S-layer fusion proteins of Geobacillus stearothermophilus NRS 2004/3a. Biomacromolecules 2010, 11, (1), 207-14. doi: 10.1021/bm901071b.

53. Messner, P.; Schäffer, C.; Egelseer, E. M.; Sleytr, U. B., Occurrence, structure, chemistry, genetics, morphogenesis and functions of S-layers. In Prokaryotic Cell Wall Compounds - Structure and Biochemistry., König, H., Claus ,H., & Varma, A. , Ed. Springer-Verlag: Berlin, 2010; pp 53–109 doi: 10.1007/978-3-642-05062-6_2.

52. Sleytr, U. B.; Egelseer, E. M.; Ilk, N.; Messner, P.; Schäffer, C.; Pum, D.; Schuster, B., Nanobiotechnological applications of S-layers. In Prokaryotic Cell Wall Compounds - Structure and Biochemistry., König, H. C., H., & Varma, A. , Ed. Springer-Verlag: Berlin, 2010; pp 459–481. doi: 10.1007/978-3-642-05062-6_16.

51. Messner, P.; Egelseer, E.-M.; Sleytr, U. B.; Schäffer, C., Surface layer proteins, secondary cell wall polymers and their glycosylation. In Microbial Glycobiology: Structures, Relevance and Applications, Moran, A.; Brennan, P.; Holst, O.; von Itzstein, M., Eds. Elsevier: San Diego, 2009; pp 109-128. doi:

50. Egelseer, E.-M.; Ilk, N.; Pum, D.; Messner, P.; Schäffer, C.; Schuster, B.; Pum, D.; Messner, P.; Schäffer, C.; Sleytr, U. B., S-Layers, microbial, biotechnological applications. . In Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology, Flickinger, M. C., Ed. John Wiley & Sons: Weinheim, Germany, 2010; pp 4424-4448. doi:

49. Zarschler, K.; Janesch, B.; Zayni, S.; Schäffer, C.; Messner, P., Construction of a gene knockout system for application in Paenibacillus alvei CCM 2051T , exemplified by the S-layer glycan biosynthesis initiation enzyme WsfP. Appl Environ Microbiol 2009, 75, (10), 3077-3085. doi: 10.1128/AEM.00087-09.

48. Thoden, J. B.; Cook, P. D.; Schäffer, C.; Messner, P.; Holden, H. M., Structural and functional studies of QdtC: an N-acetyltransferase required for the biosynthesis of dTDP-3-acetamido3,6-dideoxy-α-D-glucose. Biochemistry 2009, 48, (12), 2699-709. doi: 10.1021/bi802313n.

47. Thoden, J. B.; Schäffer, C.; Messner, P.; Holden, H. M., Structural analysis of QdtB, an aminotransferase required for the biosynthesis of dTDP-3-acetamido-3,6-dideoxy-α-D-glucose. Biochemistry 2009, 48, (7), 1553-1561. doi: 10.1021/bi8022015.

46. Steiner, K.; Wojciechowska, A.; Schaffer, C.; Naismith, J. H., Purification, crystallization and preliminary crystallographic analysis of WsaF, an essential rhamnosyltransferase from Geobacillus stearothermophilus. Acta Crystallogr Sect F Struct Biol Cryst Commun 2008, 64, (Pt 12), 1163-1165. doi: 10.1107/S1744309108036762.

45. Steiner, K.; Hanreich, A.; Kainz, B.; Hitchen, P. G.; Dell, A.; Messner, P.; Schäffer, C., Recombinant glycans on an S-layer self-assembly protein: a new dimension for nanopatterned biomaterials. Small 2008, 4, (10), 1728-40. doi: 10.1002/smll.200701215.

44. Messner, P.; Steiner, K.; Zarschler, K.; Schäffer, C., S-layer nanoglycobiology of bacteria. Carbohydr Res 2008, 343, (12), 1934-1951. doi: 10.1016/j.carres.2007.12.025.

43. Steiner, K.; Novotny, R.; Werz, D. B.; Zarschler, K.; Seeberger, P. H.; Hofinger, A.; Kosma, P.; Schäffer, C.; Messner, P., Molecular basis of S-layer glycoprotein glycan biosynthesis in Geobacillus stearothermophilus. J Biol Chem 2008, 283, (30), 21120-33. doi: 10.1074/jbc.M801833200.

42. Pföstl, A.; Zayni, S.; Hofinger, A.; Kosma, P.; Schäffer, C.; Messner, P., Biosynthesis of dTDP-3- acetamido-3,6-dideoxy-α-D-glucose. Biochem J 2008, 410, (1), 187-94. doi: 10.1042/bj20071044.

41. Tschiggerl, H.; Breitwieser, A.; de Roo, G.; Verwoerd, T.; Schäffer, C.; Sleytr, U. B., Exploitation of the S-layer self-assembly system for site directed immobilization of enzymes demonstrated for an extremophilic laminarinase from Pyrococcus furiosus. J Biotechnol 2008, 133, (3), 403-11. doi: 10.1016/j.jbiotec.2007.09.018.

40. Heinrich, M.; Schäffer, C.; Messner, P.; Allmaier, G., Negative ion ultraviolet matrix-assisted laser desorption ionization mass spectrometry and post source decay of glycosyl esters of nucleoside pyrophosphates. Eur J Mass Spectrom 2008, 14, (6), 401-9. doi: 10.1255/ejms.965.

39. Novotny, R.; Berger, H.; Schinko, T.; Messner, P.; Schäffer, C.; Strauss, J., A temperaturesensitive expression system based on the Geobacillus stearothermophilus NRS 2004/3a sgsE surface-layer gene promoter. Biotechnol Appl Biochem 2008, 49, (Pt 1), 35-40. doi: 10.1042/ba20070083.

38. Schäffer, C.; Novotny, R.; Küpcü, S.; Zayni, S.; Scheberl, A.; Friedmann, J.; Sleytr, U. B.; Messner, P., Novel biocatalysts based on S-layer self-assembly of Geobacillus stearothermophilus NRS 2004/3a: a nanobiotechnological approach. Small 2007, 3, (9), 1549- 59. doi: 10.1002/smll.200700200.

37. Bindila, L.; Steiner, K.; Schäffer, C.; Messner, P.; Mormann, M.; Peter-Katalinic, J., Sequencing of O-glycopeptides derived from an S-layer glycoprotein of Geobacillus stearothermophilus NRS 2004/3a containing up to 51 monosaccharide residues at a single glycosylation site by fourier transform ion cyclotron resonance infrared multiphoton dissociation mass spectrometry. Anal Chem 2007, 79, (9), 3271-9. doi: 10.1021/ac0617363.

36. Steiner, K.; Novotny, R.; Patel, K.; Vinogradov, E.; Whitfield, C.; Valvano, M. A.; Messner, P.; Schäffer, C., Functional characterization of the initiation enzyme of S-layer glycoprotein glycan biosynthesis in Geobacillus stearothermophilus NRS 2004/3a. J Bacteriol 2007, 189, (7), 2590-8. doi: 10.1128/jb.01592-06.

35. Zayni, S.; Steiner, K.; Pföstl, A.; Hofinger, A.; Kosma, P.; Schäffer, C.; Messner, P., The dTDP-4- dehydro-6-deoxyglucose reductase encoding fcd gene is part of the surface layer glycoprotein glycosylation gene cluster of Geobacillus tepidamans GS5-97T . Glycobiology 2007, 17, (4), 433-443. doi: 10.1093/glycob/cwl084.

34. Sleytr, U. B.; Messner, P.; Pum, D.; Schäffer, C.; Schuster, C., Molekulare Baukastensysteme. Transkript 2007, (13), 64-65. doi:

33. Steiner, K.; Pohlentz, G.; Dreisewerd, K.; Berkenkamp, S.; Messner, P.; Peter-Katalinic, J.; Schäffer, C., New insights into the glycosylation of the surface layer protein SgsE from Geobacillus stearothermophilus NRS 2004/3a. J Bacteriol 2006, 188, (22), 7914-21. doi: 10.1128/jb.00802-06.

32. Steindl, C.; Schäffer, C.; Smrečki, V.; Messner, P.; Müller, N., The secondary cell wall polymer of Geobacillus tepidamans GS5-97T : structure of different glycoforms. Carbohydr Res 2005, 340, (14), 2290-2296. doi: 10.1016/j.carres.2005.07.005.

31. Kählig, H.; Kolarich, D.; Zayni, S.; Scheberl, A.; Kosma, P.; Schäffer, C.; Messner, P., N-Acetylmuramic acid as capping element of α-D-fucose-containing S-layer glycoprotein glycans from Geobacillus tepidamans GS5-97T . J Biol Chem 2005, 280, (21), 20292-20299. doi: 10.1074/jbc.M501724200.

30. Schäffer, C.; Messner, P., The structure of secondary cell wall polymers: how Gram-positive bacteria stick their cell walls together. Microbiology 2005, 151, 643-651. doi: 10.1099/mic.0.27749-0.

29. Novotny, R.; Scheberl, A.; Giry-Laterriere, M.; Messner, P.; Schäffer, C., Gene cloning, functional expression and secretion of the S-layer protein SgsE from Geobacillus stearothermophilus NRS 2004/3a in Lactococcus lactis. FEMS Microbiol Lett 2005, 242, (1), 27-35. doi: 10.1016/j.femsle.2004.10.036. .

28. Steiner, K.; Novotny, R.; Schäffer, C.; Messner, P., Glycosylation of surface layer proteins: Glycosylation mechanisms in bacteria. In International Symposium on Downstream Processing of Genetically Engineered Antibodies and Related Molecules GE Healthcare, Amersham Biosciences AB: Uppsala, 2004; pp 41-43.

27. Schäffer, C.; Franck, W. L.; Scheberl, A.; Kosma, P.; McDermott, T. R.; Messner, P., Classification of isolates from locations in Austria and Yellowstone National Park as Geobacillus tepidamans sp. nov. Int J Syst Evol Microbiol 2004, 54, (6), 2361-2368. doi: 10.1099/ijs.0.63227-0.

26. Schäffer, C.; Messner, P., Surface-layer glycoproteins: an example for the diversity of bacterial glycosylation with promising impacts on nanobiotechnology. Glycobiology 2004, 14, (8), 31R-42R. doi: 10.1093/glycob/cwh064.

25. Novotny, R.; Schäffer, C.; Strauss, J.; Messner, P., S-layer glycan-specific loci on the chromosome of Geobacillus stearothermophilus NRS 2004/3a and dTDP-L-rhamnose biosynthesis potential of G. stearothermophilus strains. Microbiology 2004, 150, (Pt 4), 953- 65. doi: 10.1099/mic.0.26672-0.

24. Novotny, R.; Pföstl, A.; Messner, P.; Schäffer, C., Genetic organization of chromosomal S-layer glycan biosynthesis loci of Bacillaceae. Glycoconj J 2004, 20, (7-8), 435-47. doi: 10.1023/b:glyc.0000038290.74944.65.

23. Messner, P.; Schäffer, C., Prokaryotic glycoproteins. In Progress in the Chemistry of Organic Natural Products, 2003, 2, 27. ed.; Herz, W.; Falk, H.; Kirby, G. W., Eds. 2003; Vol. 85, pp 51- 124. doi:

22. Schäffer, C.; Beckedorf, A. I.; Scheberl, A.; Zayni, S.; Peter-Katalinic, J.; Messner, P., Isolation of glucocardiolipins from Geobacillus stearothermophilus NRS 2004/3a. J Bacteriol 2002, 184, (23), 6709-13. doi:

21. Steindl, C.; Schäffer, C.; Wugeditsch, T.; Graninger, M.; Matecko, I.; Müller, N.; Messner, P., The first biantennary bacterial secondary cell wall polymer and its influence on S-layer glycoprotein assembly. Biochem J 2002, 368, (Pt 2), 483-494. doi: 10.1042/BJ20020988.

20. Schäffer, C.; Wugeditsch, T.; Messner, P.; Whitfield, C., Functional expression of enterobacterial O-polysaccharide biosynthesis enzymes in Bacillus subtilis. Appl Environ Microbiol 2002, 68, (10), 4722-30. doi: 10.1128/AEM.68.10.4722-4730.2002. .

19. Schäffer, C.; Wugeditsch, T.; Kählig, H.; Scheberl, A.; Zayni, S.; Messner, P., The surface layer (S-layer) glycoprotein of Geobacillus stearothermophilus NRS 2004/3a. Analysis of its glycosylation. J Biol Chem 2002, 277, (8), 6230-6239. doi: 10.1074/jbc.M108873200.

18. Beckedorf, A. I.; Schäffer, C.; Messner, P.; Peter-Katalinic, J., Mapping and sequencing of cardiolipins from Geobacillus stearothermophilus NRS 2004/3a by positive and negative ion nanoESI-QTOF-MS and MS/MS. J Mass Spectrom 2002, 37, (10), 1086-94. doi: 10.1002/jms.369.

17. Schäffer, C.; P., M., Sweet proteins do matter. In Recent Trends in Carbohydrate Chemistry: Synthesis and Biomedical Applications of Glycans and Glycoconjugates, Rauter, A.; Christensen, B.; Somsak, L.; Kosma, P.; Adamo, R., Eds. Elsevier, Cambridge, MA, USA: 2002; Vol. 2, pp 1-36. doi:

16. Sleytr, U. B.; Sára, M.; Pum, D.; Schuster, B.; Messner, P.; Schäffer, C., Self-assembly protein systems: Microbial S-layers. In Biopolymers: Polyamides and Complex Proteinaceous Materials I, Steinbüchel, A.; Fahnestock, S., Eds. Wiley-VCH: Weinheim, Germany, 2002; Vol. 7, pp 285-338. doi:

15. Schäffer, C.; Messner, P., Glycobiology of surface layer proteins. Biochimie 2001, 83, (7), 591- 9. doi: 10.1016/s0300-9084(01)01299-8.

14. Schäffer, C.; Graninger, M.; Messner, P., Prokaryotic glycosylation. Proteomics 2001, 1, (2), 248-61. doi: 10.1002/1615-9861.

13. Schäffer, C.; Scherf, T.; Christian, R.; Kosma, P.; Zayni, S.; Messner, P.; Sharon, N., Purification and structure elucidation of the N-acetylbacillosamine-containing polysaccharide from Bacillus licheniformis ATCC 9945. Eur J Biochem 2001, 268, (3), 857-64. doi:

12. Messner, P.; Schäffer, C., Surface layer glycoproteins of Bacteria and Archaea. In Glycomicrobiology, Doyle, R. J., Ed. Plenum Press: New York, 2000; pp 93-125. doi:

11. Schäffer, C.; Müller, N.; Mandal, P. K.; Christian, R.; Zayni, S.; Messner, P., A pyrophosphate bridge links the pyruvate-containing secondary cell wall polymer of Paenibacillus alvei CCM 2051 to muramic acid. Glycoconj J 2000, 17, (10), 681-690. doi: 10.1023/A:1011062302889.

10. Schäffer, C.; Dietrich, K.; Unger, B.; Scheberl, A.; Rainey, F. A.; Kählig, H.; Messner, P., A novel type of carbohydrate-protein linkage region in the tyrosine-bound S-layer glycan of Thermoanaerobacterium thermosaccharolyticum D120-70. Eur J Biochem 2000, 267, (17), 5482-92. doi: 10.1046/j.1432-1327.2000.01610.x. .

9. Schäffer, C.; Kählig, H.; Christian, R.; Schulz, G.; Zayni, S.; Messner, P., The diacetamidodideoxyuronic-acid-containing glycan chain of Bacillus stearothermophilus NRS 2004/3a represents the secondary cell-wall polymer of wild-type B. stearothermophilus strains. Microbiology 1999, 145, (7), 1575-1583. doi: 10.1099/13500872-145-7-1575.

8. Schäffer, C.; Müller, N.; Christian, R.; Graninger, M.; Wugeditsch, T.; Scheberl, A.; Messner, P., Complete glycan structure of the S-layer glycoprotein of Aneurinibacillus thermoaerophilus GS4-97. Glycobiology 1999, 9, (4), 407-14. doi: 10.1093/glycob/9.4.407.

7. Messner, P.; Allmaier, G.; Schäffer, C.; Wugeditsch, T.; Lortal, S.; König, H.; Niemetz, R.; Dorner, M., Biochemistry of S-layers. FEMS Microbiol Rev 1997, 20, (1-2), 25-46. doi: 10.1111/j.1574-6976.1997.tb00303.x.

6. Schäffer, C.; Wugeditsch, T.; Neuninger, C.; Messner, P., Are S-layer glycoproteins and lipopolysaccharides related? Microb Drug Resist 1996, 2, (1), 17-23. doi:

5. Altman, E.; Schäffer, C.; Brisson, J.-R.; Messner, P., Isolation and characterization of an amino sugar-rich glycopeptide from the surface layer glycoprotein of Thermoanaerobacterium thermosaccharolyticum E207-71. Carbohydr Res 1996, 295, 245-253. doi:

4. Altman, E.; Schäffer, C.; Brisson, J. R.; Messner, P., Characterization of the glycan structure of a major glycopeptide from the surface layer glycoprotein of Clostridium thermosaccharolyticum E207-71. Eur J Biochem / FEBS 1995, 229, (1), 308-15. doi: 10.1111/j.1432-1033.1995.0308l.x.

3. Allmaier, G.; Schäffer, C.; Messner, P.; Rapp, U.; Mayer-Posner, F. J., Accurate determination of the molecular weight of the major surface layer protein isolated from Clostridium thermosaccharolyticum by time-of-flight mass spectrometry. J Bacteriol 1995, 177, (5), 1402- 4. doi: 10.1128/jb.177.5.1402-1404.1995.

2. Möschl, A.; Schäffer, C.; Sleytr, U. B.; Messner, P.; Christian, R.; Schulz, G., Characterization of S layer glycoproteins of two Lactobacilli. In Advances in Bacterial Paracrystalline Surface Layers, Beveridge, T. J.; Koval, S. F., Eds. Plenum Press: New York, 1993; pp 281-284.

1. Messner, P.; Schuster-Kolbe, J.; Schäffer, C.; Sleytr, U. B.; Christian, R., Glycoprotein nature of select bacterial S-layers. In Advances in Bacterial Paracrystalline Surface Layers, Beveridge, T. J.; Koval, S. F., Eds. Plenum Press: New York, 1993; Vol. 252, pp 95-107.