752339 Protein engineering
- Lecture and exercise
- Semester hours
- Lecturer (assistant)
- Offered in
- Wintersemester 2018/19
- Languages of instruction
Mutagenesis is performed with a plasmid containing the gene for pyranose 2-oxidase. After transformation in a suitable expression host (E. coli), colonies are transferred to 96 well plates, induced, grown overnight, and the cells lysed to release the mutated enzyme. This crude extracts are screened for improved thermo stability and altered substrate specificity. Mutants with improved properties are selected and sequenced.
ATTENTION: the dates given are only the reservation for the seminar room! The course is a full-day-course that will be held in the given week in this seminar room and in the Food Biotechnology research labs from 9 am until 5 pm.
- Previous knowledge expected
Knowledge of microbiology/molecular biology highly is required
- Objective (expected results of study and acquired competences)
There is an increasing demand for improved enzymes for industrial application, e.g. improved thermo stability, and altered substrate specificity. The methods of choice for producing this enzymes are directed evolution, site directed mutagenesis, gene shuffling, and a mixture of this methods. Aim of the course is an understanding of the principles of these methods, knowledge about what methods are recommended under what circumstances, and practical competence in applying one method in the lab.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.