772112 Practical course in biochemistry I


Type
Exercise course
Semester hours
5
Lecturer (assistant)
Stenitzer, David , Käfer, Maria , Kampl, Anna-Sophia , Simon, Florian , Horak, Sophia , Klier, Dominik , Köll, Alois , Nuschy, Lena , Böhmdorfer, Stefan , Wilson, Iain B.H. , Viehauser, Petra , Staudacher, Erika , Altmann, Friedrich , Föttinger, Sophie , Winter, Martina , Furtmüller, Paul Georg
Organisation
Offered in
Wintersemester 2019/20
Languages of instruction
Deutsch

Content

Quantitative determination of saccharides,

thin layer chromatography,

desalting by size exclusion chromatography,

making an enzyme extract,

dialysis,

determination of enzyme activity,

ph-and temperature dependance of an enzyme,

enzyme kinetics / determinatino of Km-value,

measurement of protein concentration,

determination of the mass of a protein by gel electrophoresis,

qualitative and quantitative analysis of biomolecules by HPLC;

.

Previous knowledge expected

Basic laboratory experience in chemistry;
Knowledge in biochemistry;

Objective (expected results of study and acquired competences)

The students know the most important basic experimental techniques in biochemistry and they are able to use them independently.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.