772328 Methods in protein characterization


Type
Lecture and exercise
Semester hours
4
Lecturer (assistant)
Traxlmayr, Michael , Schaffner, Irene , Altmann, Friedrich
Organisation
Offered in
Wintersemester 2019/20
Languages of instruction
Englisch

Content

The following topics will be covered:

UNIT 1:
Protein Quality Control with Focus on Differential Scanning Calorimetry

UNIT 2:
Introduction to flow cytometry

UNIT 3:
Affinity, avidity, binding kinetics

UNIT 4:
Cell-based assays for affinity analysis

UNIT 5:
Surface Plasmon Resonance to Determine Affinity and Kinetics of Biomolecular Interactions

UNIT 6:
More Biophysical Techniques to Study Biomolecular Interactions

UNIT 7:
Mass spectrometry (MS) techniques for biomolecules

UNIT 8:
Protein identification via MS

UNIT 9:
Quantification and analysis of posttranslational modifications via MS


HANDS-ON (please see Anmerkung/Note)

Previous knowledge expected

Basic knowledge of biochemistry, in particular protein biochemistry.

Objective (expected results of study and acquired competences)

This course will provide an overview of state-of-the-art techiques to characterize a protein sample in a comprehensive way. One focus will be on the characterization of biomolecular interactions and the available approaches to study them. The course will convey the theoretical concepts and also provide the possibility of hands-on training for selected methods (mass spectrometry, surface plasmon resonance spectroscopy, flow cytometry).
You can find more details like the schedule or information about exams on the course-page in BOKUonline.