970313 Plant metabolomics


Type
Lecture and exercise
Semester hours
4
Lecturer (assistant)
Doppler, Maria , Kluger, Bernhard , Schuhmacher, Rainer , Büschl, Christoph , Lemmens, Marc
Organisation
Offered in
Sommersemester 2022
Languages of instruction
Englisch

Content

Metabolomics is a young discipline, which deals with the global detection of the entire set of low molecular weight metabolites of a biological system (i.e. the metabolome). This course will teach the fundamentals of modern metabolomics research. The general concept will be illustrated with a practical example study on the metabolism of wheat. The lecture part will cover the basic principles of metabolism and the involved methodology. The practical section will be held in small student groups. You will carry out a complete metabolomics experiment involving
1) the treatment of wheat plants in the greenhouse
2) sampling and sample preparation
3) sample measurements by HPLC and mass spectrometry
4) data evaluation
5) biological interpretation of results

Previous knowledge expected

Basic knowledge in biochemistry, instrumental analytical chemistry and statistics

Objective (expected results of study and acquired competences)

You will learn the general concept and how to setup a metabolomics study. The basics of plant biochemistry, HPLC and mass spectrometry will be refreshed and deepened. The practical training will enable to experience all steps of a metabolomics study. Summarizing, interpreting and presenting the results will get acquainted with critically assessing the outcome of a scientific experiment.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.