772311 Kinetics of biochemical reactions

Type
Lecture and exercise
Semester hours
2
Lecturer (assistant)
Organisation
Offered in
Sommersemester 2023
Languages of instruction
Englisch
Content

Basics of biochemical Reactions: enzyme classification, thermodynamic basis of biochemical reactions, reaction order, pH and temperature dependence of enzymes.

Derivation of the Michaelis-Menten equation: analysis of enzyme kinetic data, graphical representations of the Michaelis-Menten equation, linearization methods, derivation of rate equation for reversible enzyme reactions, the Haldane relationship, examples.

Enzyme inhibition: unspecific enzyme inhibition, irreversible enzyme inhibition, suicide substrates, analysis of irreversible inhibitions, reversible enzyme inhibition, derivation of general rate equation for inhibition, graphical representations of enzyme inhibition, examples.

Ligand binding: interaction between macromolecules and ligands, general binding equation, graphic representations of the binding equations, cooperativity, the Hill equation, allosteric enzymes, physiological aspects of cooperativity, examples.

Mechanisms with more than two substrates: nomenclature, random mechanism, ordered mechanism, ping-pong mechanism, specific mechanism, derivation of general rate equation, examples.

Pre-steady-state kinetics: deviation of basic equation, evaluation of rapid kinetic reactions (transient kinetics), methods for measuring pre-steady-state kinetics, flow methods, relaxation methods, flash photolysis.

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Objective (expected results of study and acquired competences)

After successful attendance of this lecture the students should be familiar with the basics of biochemical reactions like reaction order or thermodynamic aspects. The student can derive the Michaelis-Menten equation on different examples and the can determine and evaluate the Michaelis-Menten parameters. On the basis of selected examples the students can differentiate between selected inhibitions pattern and can calculate and evaluate the inhibition constants. The students can describe cooperativity and different allosteric models. The students are able to plan and make reactions with more than one substrate and evaluate the derived kinetic parameters. They should know common methods and analysis for measuring rate constants and they should be able to evaluate if they have selected the right method.

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You can find more details like the schedule or information about exams on the course-page in BOKUonline.