790327 Practical course in plant biotechnology
- Type
- Exercise course
- Semester hours
- 3
- Lecturer (assistant)
- Organisation
- Offered in
- Sommersemester 2023
- Languages of instruction
- Englisch
- Content
-
Preparation of culture media, working under aseptic conditions; surface sterilization and preparation of the source isolate (tissue); initiation and passaging of callus cultures and suspension cultures, explants; isolation of protoplasts; exercises to study the influence of periphysis, cyclophysis and topophysis on the establishment of cultures, mediated transformation by means of Agrobacterium, GUS assay
- Previous knowledge expected
-
791111 Plant Biotechnology
- Objective (expected results of study and acquired competences)
-
Students will be experienced in the practical implementation of the techniques of in-vitro culture and protein analyses:
+ know how to establish, maintain, propagate different samples of plant tissues in vitro
+ handle the transfer to glass house conditions
+ be able to induce somatic embryogenesis
+ carry out transformation experiments
+ verify the transgenic status of plant material
+ analyse independently plant (fruit ) samples for the presence of allergens
You can find more details like the schedule or information about exams on the course-page in BOKUonline.