940336 Exercises in molecular biology


Type
Practical course
Semester hours
3
Lecturer (assistant)
Fischer, Marzena , Lucyshyn, Doris , Luschnig, Christian
Organisation
Offered in
Wintersemester 2022/23
Languages of instruction
Englisch

Content

induced gene expression in plants, analysis of the resulting plant phenotypes, quantitative real-time PCR, Western blot analysis, reporter gene analysis in whole plants, Confocal Laser Scanning microscopy,

Previous knowledge expected

theoretical background of molecular biology essential, practical experiences in molecular biological techniques are welcome.

Objective (expected results of study and acquired competences)

After completion of this course, students will be able to isolate RNA and proteins from plant tissue. Furthermore, they will be able to determine RNA/protein sample concentrations and will be familiarized with general aspects of gene expression control and its analysis in plants. In addition, students will be capable to perform basics of cell/biochemical techniques, involving Confocal Laser Scanning Microscopy (CLSM), Western blotting and quantitative Real Time PCR.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.