940336 Exercises in molecular biology
- Type
- Practical course
- Semester hours
- 3
- Lecturer (assistant)
- Organisation
- Offered in
- Wintersemester 2022/23
- Languages of instruction
- Englisch
- Content
-
induced gene expression in plants, analysis of the resulting plant phenotypes, quantitative real-time PCR, Western blot analysis, reporter gene analysis in whole plants, Confocal Laser Scanning microscopy,
- Previous knowledge expected
-
theoretical background of molecular biology essential, practical experiences in molecular biological techniques are welcome.
- Objective (expected results of study and acquired competences)
-
After completion of this course, students will be able to isolate RNA and proteins from plant tissue. Furthermore, they will be able to determine RNA/protein sample concentrations and will be familiarized with general aspects of gene expression control and its analysis in plants. In addition, students will be capable to perform basics of cell/biochemical techniques, involving Confocal Laser Scanning Microscopy (CLSM), Western blotting and quantitative Real Time PCR.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.