752311 Biochemical reaction engineering
- Type
- Lecture
- Semester hours
- 2
- Lecturer (assistant)
- Organisation
- Offered in
- Wintersemester 2022/23
- Languages of instruction
- Englisch
- Content
-
The central theme of this lecture is the application of enzymes in biocatalysis and the design of an enzymatic process. The following aspects of biochemical reaction engineering will be discussed:
1. Course overview and introduction
2. Biotechnology, biotransformations, catalysis,…
2.1. Additional reading – enzyme evolution
3. Biocatalysis
4. Enzyme applications
4.1. Additional reading – flavoprotein oxidases
4.2. Additional reading – C-C hydrolases
5. Exercise 1
6. Biochemical reaction engineering, biocatalyst screening and engineering
6.1. Additional reading – Accelerating the implementation of biocatalysts in industry
7. Enzymatic activity, diffusion, collisions and transitions
8. Enzyme kinetics (microkinetics)
8.1. Additional reading – Die Kinetik der Invertinwirkung
9. Inhibition, multi-reactant systems
10. Substrate specificity, enantioselectivity,…
11. Exercise 2
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12. Macrokinetics
12.1. Additional reading – considerations when measuring biocatalyst performance
13. Reactor types
13.1. Additional reading – cascading microreactors
13.2. Additional reading – flow bioreactors
14. Scale-up, process control, process monitoring
15. Exercise 3
16. Enzymatic redox transformations
16.1. Additional reading – multi-enzyme processes
17. Exercise 4
18. Immobilisation vs. enzyme membrane reactor concept
18.1. Additional reading – immobilized enzymes
19. Enzyme stability and deactivation
19.1. Additional reading – kinetic stability of proteins
19.2. Additional reading – calulating TTN in continuous processes
20. Solvents and environmental impact of processes
20.1. Additional reading – on the relationship between enantioselectivity and enzyme
flexibility
20.2. Additional reading – enzyme-catalyzed processes in organic solvents
21. Green chemistry
21.1. Additional reading – catalysis, green chemistry and process design
21.2. Additional reading – E-factors
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Please check BOKUlearn for more information and the Zoom links.
- Previous knowledge expected
-
Participants will have to recall their knowledge from different lectures and disciplines in the field of biotechnology and create new connections.
- Objective (expected results of study and acquired competences)
-
After the positive completion of the course the students can:
1) Describe essential concepts of reaction kinetics (mass balance, mass transfer) and reactor types
2) Recall and explain important catalytic constants (Km, kcat,...) and process parameters (T, pH, [S], [P], [I],,...) which affect enzymatic reactions and their applications in industrial scale processes
3) Derive and interprete process diagrams (substrate conversion, product formation, reaction rate,...) and performance numbers (space-time yield, specific productivity, total turnover number,...)
4) Apply fundamental principles of biocatalysis for the engineering of a biochemical reaction
5) Asses and argue the influence of catalytic constants on enzymatic processes
6) Generate solutions for possible problems caused by non-ideal enzymes and poor reaction conditions
You can find more details like the schedule or information about exams on the course-page in BOKUonline.