790327 Practical course in plant biotechnology

Exercise course
Semester hours
Lecturer (assistant)
Borroto Fernandez, Eduviges Glenda , Laimer, Margit
Offered in
Sommersemester 2024
Languages of instruction


Preparation of culture media, working under aseptic conditions; surface sterilization and preparation of the source isolate (tissue); initiation and passaging of callus cultures and suspension cultures, explants; isolation of protoplasts; exercises to study the influence of periphysis, cyclophysis and topophysis on the establishment of cultures, mediated transformation by means of Agrobacterium, GUS assay

Previous knowledge expected

791111 Plant Biotechnology

Objective (expected results of study and acquired competences)

Students will be experienced in the practical implementation of the techniques of in-vitro culture and protein analyses:
+ know how to establish, maintain, propagate different samples of plant tissues in vitro
+ handle the transfer to glass house conditions
+ be able to induce somatic embryogenesis
+ carry out transformation experiments
+ verify the transgenic status of plant material
+ analyse independently plant (fruit ) samples for the presence of allergens
You can find more details like the schedule or information about exams on the course-page in BOKUonline.