790417 Instructional course IVC - protein purification
- Type
- Exercise course
- Semester hours
- 2
- Lecturer (assistant)
- Jungbauer, Alois
- Organisation
- Offered in
- Sommersemester 2024
- Languages of instruction
- Englisch
- Content
-
An introduction to the governing principles of preparative chromatography, membrane filtration and precipitation/flocculation with respect to separation of proteins and other macromolecules will be given. Relevant methods will be described for the purification of proteins from different starting materials such as microbial cells, mammalian cells and plant tissue, in particular when a purification tag is not available. Also a detailed insight into available purification tags will be provided, and their advantages and disadvantages will be discussed.
- Previous knowledge expected
-
None
- Objective (expected results of study and acquired competences)
-
The course will provide instructions on how to optimize a purification scheme, set-up a de novo purification protocol without prior knowledge of protein properties and how to scale up a protein purification method. The students will be trained in trouble shooting of chromatography, membrane filtration, precipitation/flocculation and strategies to avoid protein degradation during purification. Students will be able to select purification tags on a rational basis
You can find more details like the schedule or information about exams on the course-page in BOKUonline.