953306 Laboratory diagnosis
- Type
- Exercise course
- Semester hours
- 2
- Lecturer (assistant)
- Gasser, Katharina , Laure, Nicole , Bersch, Lana , Wieczorek, Krzysztof
- Organisation
- Offered in
- Sommersemester 2024
- Languages of instruction
- Deutsch
- Content
-
Short overview of the protein extraction from plant tissue and protein detection using specific antibodies: ELISA immunoassay to detect plant viruses in plant tissues.
Short overview of the extraction of DNA/RNA from plant tissue and RNA/DNA detection: RT-PCR-based detection of plant viruses.
DNA fingerprinting of different Phytophthora spp.: PCR and digestion of the PCR product with restriction enzymes. DNA detection via gel electrophoresis.
In addition to the experiments that will be conducted by the students basic background information will be given concerning the different detection methods.
- Previous knowledge expected
-
no practical experience necessary
- Objective (expected results of study and acquired competences)
-
Basic knowledge of antibody-based ELISA test as a tool to detect plant pathogenes.
Basic knowledge of PCR-based detection of plant pathogenes.
Students can perform ELISA test and PCR to detect plant pathogenes.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.