953327 Laboratory diagnosis of plant damages

Exercise course
Semester hours
Lecturer (assistant)
Hage-Ahmed, Karin , Wieczorek, Krzysztof , Kronberger, Markus , Gasser, Katharina
Offered in
Wintersemester 2023/24
Languages of instruction


Short overview of the protein extraction from plant tissue and protein detection using specific antibodies: ELISA immunoassay to detect plant viruses in plant tissues.
Short overview of the extraction of RNA from plant tissue and virus RNA detection using RT-PCR-based method.
DNA fingerprinting of different strains of phytopathogenic fungi.: PCR and digestion of the PCR product with restriction enzymes. DNA detection via gel electrophoresis.

In addition to the experiments that will be conducted by the students basic background information will be given concerning the different detection methods.

Previous knowledge expected

no practical experience necessary

Objective (expected results of study and acquired competences)

- Basic knowledge of antibody-based ELISA test as a tool to detect plant pathogens.
- Basic knowledge of PCR-based detection of plant pathogens.
- Students can perform ELISA test and PCR to detect plant pathogens.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.