790037 Characterisation of blood stem cells as starting cells for biotechnology

Type
Lecture and seminar
Semester hours
1
Lecturer (assistant)
Henschler, Reinhard , Kasper, Cornelia
Organisation
Offered in
Wintersemester 2023/24
Languages of instruction
Englisch
Content

1.Motivation:
a.Stem Cells as Starting Cells for Tissue Regeneration
b.Utilization of Stem Cell Properties in Medicine, exemplified by treatment methods in clinical hemato-Oncology
2.Basics of Hematopoetic Stem Cell Biology
a.Terms and Definitions
b.Measurement and Visualization of stem cells via their daughter cells / progeny in vitro und in vivo
c.Phenotypic Characterisation
d.Developmental Biology Aspects
e.Role of Support Cells (Stroma, Nurse Cells)
3.Utilization of Stem Cells in Biotechnology
a.Concept of the Stem Cell Niche
b.Significance of Culture Media
c.Signals for Differentiation
d.Geometrical Aspects for Cell Seeding and Cell Separation
4.Bioreaktors for Stem Cell Cultivation
a.Model of the Classic Long Term Bone Marrow Culture
b.Copying Biological Stem Cell Niches in Bioreactors: Design and Qualification of Materials, Flow Dynamics, Media, 3D Cultures versus 2D
c.Methods to Monitor Culture Development over Time
d.Description of the Desired End Products
5.Needs for Adaptation to Use in Clinical Medicine:
a.Good Manufacturing Practice (GMP)
b.Spezifications of the Starting Materials and the Final Product
c.Examples in Current Clinical Studies
6.Determination of Hematopoetic Cell Types
a.Example of a Stem Cell Analysis by Flow Cytometry (Practical)
b.Other Methodologies
Previous knowledge expected

Basic knowledge in cell biology and biotechnology and bioprocess engineering
Bachelor in LBT or similar study
Objective (expected results of study and acquired competences)

The Course Participants will
-understand the theoretical background of stem cell biology, and the history of the discovery of primitive hematopoietic as well as hematopoietic stem cells
-know the principles of long term bone marrow culture and its value for analyzing the presence and potency of hematopoetic stem cells
-understand and perform simple flow cytometric analysis of blood cells
-know 2 problems on advancing stem cell transplantation to use of cultured stem cells, which are currently being solved and brought into the clinic.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.