940402 Basic course II - biosynthesis, post-translational modifications and trafficking of recombinant proteins

Type
Lecture
Semester hours
2
Lecturer (assistant)
Gasser, Brigitte , Stadlmann, Johannes , Stöger, Eva , Bobbili, Madhusudhan Reddy , Wilson, Iain B.H. , Strasser, Richard
Organisation
Offered in
Sommersemester 2025
Languages of instruction
Englisch
Content

Part A: Biosynthesis and posttranslational modifications of recombinant proteins

Biosynthesis of cytosolic and secretory proteins
Protein folding, quality control and co-factor incorporation (heme, flavin) within the cytosol and the secretory pathway
Posttranslational protein modifications I: N- and O-glycosylation in biotechnologically relevant organisms
Posttranslational protein modifications II: role of glycosylation in targeting, turnover and immunology of recombinant proteins
Posttranslational protein modifications III: phosphorylation, hydroxylation, carboxylation, acetylation, methylation etc. and their functional relevance
Analysis of post-translational modifications I: chromatographic and electrophoretic techniques
Analysis of post-translational modifications II: mass spectrometry
Proteomics: concepts and recent developments


Part B: Intracellular protein trafficking and its biotechnological relevance

General concepts of intracellular transport processes
The secretory pathway I: the endoplasmic reticulum
The secretory pathway II: the Golgi apparatus
Protein secretion in yeasts and filamentous fungi
Regulated and non-canonical secretion
Endocytosis, endosomes and lysosomes
Intracellular protein degradation pathways
Protein storage organelles
.
Previous knowledge expected

Basic knowledge in protein biochemistry and molecular cell biology.
Objective (expected results of study and acquired competences)

Improved knowledge of protein biosynthesis and post-translational protein modifications and their functionalities. Profound understanding of the relevance of post-translational protein modifications for the production and application of recombinant proteins. Comprehensive knowledge of eukaryotic expression systems with respect to protein secretion, endocytosis and turnover.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.