774305 Plant polysaccharide analysis

Type
Lecture
Semester hours
2
Lecturer (assistant)
Hettegger, Hubert , Sulaeva, Irina
Organisation
Offered in
Sommersemester 2025
Languages of instruction
Englisch
Content

Part 1: Raw materials from plants - important polymers and their isolation:
•Introduction to the chemistry of carbohydrates
•Overview of important raw materials from plants
•Brief digression on lignin - another important biopolymer
•Classification of their role in plants and how to benefit from them
•Technology of isolation
•Modification and biological degradation

Part 2: Analysis - important properties and how to determine them:
•Immunofluorescence analysis
•Hydrolysis and methanolysis for monosaccharide analysis
•Approaches for derivatization
•GC/MS, HPLC, HPTLC
•Qualitative and quantitative analysis of substitution (DS)
•Determination of the molar mass of polysaccharides
•GPC, SEC, FFF, viscometry
•IR spectroscopy (FTIR), UV/Vis and fluorescence spectroscopy
•NMR methods for structural analysis
•Optical properties, electron microscopy
•Elemental analysis
Previous knowledge expected

The students have basic knowledge of organic and polysaccharide chemistry. Preferably, the students have participated in 774314 "Biopolymers for sustainable utilization" or equivalent lectures.
Objective (expected results of study and acquired competences)

The students are able to discuss the process from raw material via isolation and modification to analytical procedures that are appropriate to describe changes inflicted by the respective procedures. They can evaluate appropriate analytical techniques to acquire the properties of the respective polysaccharide, e.g. cellulose or starch, and know how to present and how to discuss and interpretate analytical results.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.