953318 Gene technology for plant pathologists
- Type
- Lecture
- Semester hours
- 2
- Lecturer (assistant)
- Wieczorek, Krzysztof , Hage-Ahmed, Karin
- Organisation
- Offered in
- Wintersemester 2024/25
- Languages of instruction
- Englisch
- Content
-
Gene technology for plant pathologists lecture deals with different molecular biology aspects and methods currently relevant in the crop production and protection such as: plant resistance, cloning, production and use of transgenic plants to improve their resistance against pathogens and pests, different methods of plant genome engineering etc.
- Previous knowledge expected
-
- Objective (expected results of study and acquired competences)
-
Students gain knowledge of:
- Basic genetic cloning methods including PCR, restriction enzymes, different plasmids and vectors etc.
- Plant transformation tools, selection markers and reporter genes.
- Examples of transgenic plants in the field.
- Resistance genes and host resistance.
- Technics of plant genome engineering including RNAi, ZFN, TALENs and CRISP/Cas9.
- Mechanisms of plant resistance against different pests and pathogens including induced resistance, systemic acquired resistance, induced systemic resistance, ETI and PTI etc.
- The role of secondary metabolites in the plant resistance.
Learning outcomes:
Upon completion of this course, the students will be able to:
- name and understand the principles of gene cloning including the PCR, digestion with restriction enzymes, ligation and selection of transformed bacteria using different systems
- name and understand the different plant resistant mechanisms including induced resistance, systemic acquired resistance, induced systemic resistance, ETI and PTI etc.
- name and understand different methods of plant genome engineering
- name and understand the role of different substances in the plant resistance such as phytoalexins, phytoanticipins and different secondary metabolites
- name and understand transformation tools such as Agrobacterium-mediated transformation and their role in plant-pathogen studies
- name and understand examples of selection markers and reporter genes
- name and understand examples of transgenic plants in the field and assess their advantages and disadvantages
- name and understand principles of resistance genes, classes of resistance genes and the location of the corresponding proteins
- name and understand principles of perception of pathogen effectors in plants and host resistance
You can find more details like the schedule or information about exams on the course-page in BOKUonline.