790397 Protein engineering
- Type
- Lecture
- Semester hours
- 3
- Lecturer (assistant)
- Wozniak-Knopp, Gordana , Peterbauer, Clemens Karl , Wick, Cecilia
- Organisation
- Offered in
- Wintersemester 2024/25
- Languages of instruction
- Englisch
- Content
-
•Antibodies
•Vaccines
•Influenza / HIV / HPV
•Difficult-to-express proteins
•Fusion proteins
•Recombinant Allergens
•High throughput screening / microfluidics
•Enzyme evolution
•Technology of library construction
•Design of alternative binding scaffolds
- Previous knowledge expected
-
Basic knowledge in molecular biology and biochemistry
Protein structure and function
- Objective (expected results of study and acquired competences)
-
Lecture 1: Antibodies
Learning objectives:
-To describe the antibody fold, antibody domain structure and molecular architecture
-To define the beneficial biological properties of antibodies and relate them to molecular structure
-To comment on the importance of antibody-based therapeutics in present times
Lectures 2 and 3: anti-viral Vaccines
Learning objectives:
-To describe various approaches how protein engineering helps to design better vaccines
-To introduce rational, computational, and combinatorial strategies for vaccine design
-To define the requirements for successful vaccines and how these can be met by protein engineering approaches
-To discuss and understand selected examples for vaccines that are based on protein engineering
Lecture 4: Difficult-to-express proteins
Learning objectives:
-To describe the properties of Difficult-to-express proteins as well as their role as therapeutic targets
-To evaluate various approaches and methods to improve solubility, folding, enable crystallization and prevent aggregation and formation of inclusion bodies
Lecture 5: Fusion proteins
Learning objectives:
-To comment on the advantages of fusion proteins intended for therapeutic purposes, bioanalytics and industrial applications
-To compare different designs of fusion proteins and corresponding tags
-To discuss challenges in expression, production and analytics of fusion proteins
Lecture 6: Recombinant Allergens
Learning objectives:
-To describe and compare the molecular structures of allergens
-To comment on molecular allergen immunotherapy vs. allergen extract based AIT
-To deliver an up to date view of recombinant allergens for treatment and allergen-specific prevention
Lecture 7: High throughput screening / microfluidics
Learning objectives:
- To understand the principal characteristics of enzyme activity assays: localization of enzyme, nature of substrate and product, detectable and quantifyable molecules
- Knowledge of factors that influence the detection of a product/byproduct/secondary product of an enzymatic reaction and its link to the genotype
Lecture 8: Enzyme evolution
Learning objectives:
- To understand the drivers of evolution of enzyme activity: mutation and recombination, selective pressure, trade-offs between stability and dynamics
- To describe the acquisition of novel functions and reactivities
Lecture 9 and 10: Technology of library construction
Learning objectives:
-To describe the concept of library display and selection
-To compare various library display systems
-To choose a proper mutagenesis method for the intended protein modification
-To design a selection process protocol for the desired change of protein function
Lecture 11: Design of alternative binding scaffolds
Learning objectives:
-To identify the key properties of a functional binding scaffold
-To implement the knowledge on protein structure in designing a binding surface
-To apply the principles of library selection methods to the design of a binding scaffold
You can find more details like the schedule or information about exams on the course-page in BOKUonline.