940115 Laboratory course in molecular biology I

Type: Exercise course
Semester hours: 3
Lecturer (assistant): Kohlfürst, Elisabeth , Amberger, Lena , Gradischnig, Elias , Demuth, Martina , Riedel, Niklas , Tremel, Patrik , Schierer, Jakob , Kunnummel, Vinny , Luschnig, Christian , Schoberer, Jennifer , Strasser, Richard , Grabherr, Reingard , Hauser, Marie-Theres , Klausberger, Miriam
Organisation
Offered in: Wintersemester 2024/25
Languages of instruction: Englisch
Content: for details see BOKUlearn

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Previous knowledge expected: 940105 Introduction to Molecular Biology
790128 Allgemeine Mikrobiologie Übungen
Objective (expected results of study and acquired competences): By the end of the training the students will

- understand the basic principles of DNA cloning

- be able to perform basic methods in molecular biology including restriction enzyme digestion, ligation, transformation of bacteria, PCR, plasmid preparation, monitoring of gene expression, purification of genomic DNA, Western Blot hybridisation

- be aware of the theoretical background of the different molecular biology methods

- understand principles of DNA-sequencing technologies

- be able to apply quantitative PCR to determine the gene copy number

- be able to critically interpret experimental data

You can find more details like the schedule or information about exams on the course-page in BOKUonline.