BTLW300948 Strain improvement of microorganisms and higher eukaryotic cells

Type
Lecture and seminar
Semester hours
2
Lecturer (assistant)
Hernandez-Bort, Juan-Antonio , Kunert, Renate , Üzülmez, Öykü
Organisation
Biotechnology and Food Science
Offered in
Sommersemester 2026
Languages of instruction
Englisch
Content

Based on work done during contact hours and seminars, the various options for optimizing recombinant gene expression are explored.
Six topics are covered in nine teaching units. Three units per supervisor.
Additionally, we will define the subjects for the final presentation of each student which are held in the according lecture.
Students must attend at least six teaching units (135-minutes each unit) out of nine, to graduate.
The minimum number of students is five.
Here are six topics that will be covered through discussion of the relevant literature.
•Host cell engineering in animal cells: glycosylation, reduction of apoptosis - Juan Hernandez-Bort
•Host cell engineering of E. coli: BL21, K-12, etc. Expression in IBs vs. secreted - Öykü Üzülmez
•Gene transfer in bacteria: conjugation-transformation-transduction - Renate Kunert
•Gene transfer in yeast: transformation, selection marker, optimization of the gene of interest - Renate Kunert
•Gene transfer in plants: direct, indirect, expression hosts in vivo and in vitro - Öykü Üzülmez
•Gene transfer in mammalian cells: transfection, transduction, infection, cell fusion Juan Hernandez-Bort
Previous knowledge expected

Knowledge of the content of the course “Cell and Molecular Biology” or the course “Cell Factories.”
Objective (expected results of study and acquired competences)

Overview on current scientific methods and their combination for the generation of biotechnological production clones.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.