NWNR300892 Methods in protein characterization

Type
Lecture and exercise
Semester hours
4
Lecturer (assistant)
Laurent, Elisabeth , Traxlmayr, Michael , Grünwald-Gruber, Clemens , Schaffner, Irene
Organisation
Biochemistry
Offered in
Wintersemester 2026/27
Languages of instruction
Englisch
Content

The following topics will be covered:

UNIT 1:
BOKU Core Facilities and Protein Quality Control

UNIT 2:
Surface Plasmon Resonance (SPR) to Determine Affinity and Kinetics of Biomolecular Interactions

UNIT 3:
Additional Biophysical Techniques for Studying Biomolecular Interactions

UNIT 4:
Introduction to Flow Cytometry

UNIT 5:
Affinity, Avidity & Binding Kinetics

UNIT 6:
Cell-Based Assays for Affinity Analysis

UNIT 7:
Mass spectrometry (MS) for Biomolecular Analysis

UNIT 8:
Protein Identification by MS

UNIT 9:
Quantification and Analysis of Post-Translational Modifications by MS

HANDS-ON (please see Anmerkung/Note)
Previous knowledge expected

Basic knowledge of biochemistry, with a particular focus on protein biochemistry.
Objective (expected results of study and acquired competences)

This course provides an overview of state-of-the-art techniques for the comprehensive characterization of protein samples. A particular emphasis will be placed on biomolecular interactions, including their characterization and the different approaches available for studying affinity, binding kinetics, and related properties. The course combines theoretical concepts with hands-on training in selected methods, including mass spectrometry, surface plasmon resonance spectroscopy, and flow cytometry.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.