BTLW401008 Instructional course IIIA - animal cell culture and technology (in Eng.)
- Type
- Exercise course
- Semester hours
- 2
- Lecturer (assistant)
- Virgolini, Nikolaus , Borth, Nicole
- Organisation
- Biotechnology and Food Science
- Offered in
- Wintersemester 2026/27
- Languages of instruction
- Englisch
- Content
-
Routine cell culture of CHO cells
- cell density measurement
- calculation of seeding density
- passaging
- calculation of growth rate
CRISPR/Cas9 editing:
- Transfection of CHO cells with plasmids coding for CRISPR/Cas9
- Analysis of transfection efficiency with flow cytometry
- Genotyping of DNA edits
- Staining and flow cytometry analysis of phenotypic effect
- Discussion and interpretation of results
- Objective (expected results of study and acquired competences)
-
After completion of this course students are familiar with handling of animal cell cultures and associated advanced technologies. They understand the potential and capacity of animal cell systems for the production of recombinant proteins and they can engineer cell line for specific advantageous behaviour.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.