High throughput bioactivity screening
The unit provides HT biomonitoring with an assembly of an automatic incubator (Cytomat 42) and a robotic arm (Hamilton Rackrunner) in combination with a multiwell Reader (Synergy H1). Thus, microbial growth or other parameters can be measured in parallel in up to 42 96-well plates over time. The recorded growth curves provide additional quality analytic means to quantify growth of microorganisms. We routinely use several species of bacteria, yeast and candida as well as filamentous fungi and Arabidopsis as target organisms. Possible readouts include absorbance, fluorescence, and luminescence. Therefore, detection of many parameters can be performed; from enzyme activities to gene expression in vivo.
Isolation of bioactive Compounds
We have equipment for incubation, upscaling and isolation of bioactive compounds. Incubators to control a wide temperature range, light, CO2. For larger scale we offer incubation rooms with incubator racks. For purification of bioactive compounds we use various cell disruption techniques (FastPrep 96), extraction and Flash chromatography, Rotavapor and Prep HPLC. Chemical identification may be carried out by the analysis part of the CF.
Logistics and tracking
All pipetting operations are recorded and all devices are equipped with bar code readers. We use complete semiautomatic assignment of the manipulated Labware. A complete data tracking can also be maintained throughout the device group with all combined processes.
