940336 Exercises in molecular biology


Type
Practical course
Semester hours
3
Lecturer (assistant)
Luschnig, Christian , Lucyshyn, Doris , Fischer, Marzena
Organisation
Offered in
Wintersemester 2021/22
Languages of instruction
Englisch

Content

Getting to know reporter constructs in plants and their use. Analysis of induced gene expression of reporter constructs: phenotypic impact, quantitative real-time PCR, Western blot, subcellular localization of proteins in plant cells (confocal laser scanning microscopy)

Previous knowledge expected

Theoretical background of molecular biology essential, practical experiences in molecular biological techniques are welcome.

Objective (expected results of study and acquired competences)

Upon completion of this course, students will understand the principle of reporter constructs and how they are applied in plant science. They will know basic aspects of the control of gene expression in plants, and their analysis, and will be able to apply various methods by which gene expression can be measured or observed. Skills acquired include extraction, quantification and quality control of RNA and proteins from seedlings, qPCR and Western blot, and phenotyping of seedlings. Students will also receive an introduction to confocal microscopy of plant cells and analysis of subcellular localization in plant cells.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.