940341 Molecular genetics of baker´s yeast

Lecture and exercise
Semester hours
Lecturer (assistant)
Offered in
Wintersemester 2022/23
Languages of instruction


Yeast: classical genetics, yeast transformation, vector types, transformation markers, expression signals, 2-hybrid system and derivatives, regulatable promoters, proteasome, prions in yeast.

Genomics: use of genome databases for yeast and sequenced fungi (MIPS, SGD)

In the practical part of the course specific topics from the theoretical part will be applied: yeast media will be prepared and methods such as transformation, genomic DNA isolation, Y2H system, crosses, sporulation, etc. will be performed.

Previous knowledge expected

basic knowledge in geneics and biochemistry

Objective (expected results of study and acquired competences)

The participants should get an overview about methods and techniques for genetic manipulation of yeast, with a focus on the use of the yeast 2-hybrid system.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.