772304 Protein chemistry and protein engineering

Type
Lecture and exercise
Semester hours
3
Lecturer (assistant)
Zajc, Charlotte , Oostenbrink, Chris , Furtmüller, Paul Georg , Peterbauer, Clemens Karl , Ludwig, Roland , Wozniak-Knopp, Gordana
Organisation
Offered in
Sommersemester 2024
Languages of instruction
Englisch
Content

THEORETICAL PART - LECTURES

Unit I: Protein chemistry I: Forces between molecules and binding energies of molecules, structural building blocks, motifs and (super) secondary structures: OBINGER

Unit II: Protein chemistry II: Assembly from the building blocks, tertiary and quaternary structures, protein diversity and classification, databases and protein visualization: OBINGER

Unit III: High-resolution structure determination of proteins (X-ray, NMR): OBINGER

Unit IV: Protein analysis & quality control I: Low resolution structure determination (CD, IR, fluorescence): OBINGER

Unit V: Protein analysis & quality control II: Conformational and thermal stability, mono/polydispersity ect. (calorimetry, light scattering techniques): OBINGER

Unit VI: Protein dynamics and molecular dynamics simulations: OOSTENBRINK

Unit VII: Protein engineering I/mutagenesis strategies: RÜKER/PETERBAUER

Unit VIII: Protein engineering II/display techniques and screening: RÜKER/PETERBAUER

Unit IX: Protein engineering III/examples from antibody engineering & mechanism based-enzyme design: RÜKER/PETERBAUER

Unit X: Functional studies on proteins I: Enzyme kinetics (steady-state versus presteady kinetics): FURTMÜLLER

Unit XI: Functional studies on proteins II: Ligand binding and protein-protein interaction (surface plasmon resonance, biolayer interferometry ect.): FURTMÜLLER



PRACTICAL PART:

Databases and phylogeny: LUDWIG

Protein structure modeling and prediction: OOSTENBRINK

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Previous knowledge expected

Basic lectures in Organic Chemistry, Biochemistry and Molecular Biology
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Objective (expected results of study and acquired competences)

After completing this course and passing the exam the student should understand the relationship between protein structure and function as well as protein dynamics and stability. Moreover she/he will have an overview about the most important techniques in elucidation of protein structure and quality. The student will know the principles in protein design, engineering and selection as well as the use of the most important databases, elucidation of protein phylogeny as well as the basics in protein modeling and structure prediction.
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You can find more details like the schedule or information about exams on the course-page in BOKUonline.