940413 Instructional course IIC - cell imaging
- Type
- Exercise course
- Semester hours
- 2
- Lecturer (assistant)
- Reimhult, Erik , Dünser, Kai Alexander , Arcalis, Elsa , Schoberer, Jennifer
- Organisation
- Offered in
- Sommersemester 2024
- Languages of instruction
- Englisch
- Content
-
Use of fluorescent protein tags as reporters for subcellular localization, expression of fluorescently-tagged marker proteins in different subcellular compartments in plants and mammalian cells. Methods to be applied include live-cell imaging, confocal laser scanning microscopy, quantitative co-localization studies, measurement of protein dynamics using fluorescence recovery after photobleaching or photoactivatable fluorescent proteins, monitoring of in vivo protein-protein interactions using fluorescent protein techniques like biomolecular fluorescence complementation.
- Objective (expected results of study and acquired competences)
-
Students will be familiar with basic and cutting edge light microscopy techniques. Increased understanding of the subcellular organization and secretory pathway of eukaryotic cells.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.