940413 Instructional course IIC - cell imaging (in Eng.)
- Art
- Übung
- Semesterstunden
- 2
- Vortragende/r (Mitwirkende/r)
- Arcalis, Elsa , Schoberer, Jennifer , Schäffer, Christina
- Organisation
- Angeboten im Semester
- Sommersemester 2024
- Unterrichts-/ Lehrsprachen
- Englisch
- Lehrinhalt
-
Use of fluorescent protein tags as reporters for subcellular localization, expression of fluorescently-tagged marker proteins in different subcellular compartments in plants and mammalian cells. Localisation and tracking of proteins to follow subcellular trafficking.
Methods to be applied include live-cell imaging, confocal laser scanning microscopy, quantitative co-localization studies, measurement of protein dynamics using fluorescence recovery after photobleaching or photoactivatable fluorescent proteins, monitoring of in vivo protein-protein interactions using fluorescent protein techniques like biomolecular fluorescence complementation. Fixation and embedding of samples for electron microscopy, ultramicrotomy, basic electron microscopy methods, negative staining, immunolocalisation methods, electron tomography.
- Lehrziel
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Students will be familiar with basic and cutting edge light and electron microscopy techniques. Increased understanding of the subcellular organization and secretory pathway of eukaryotic cells.
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finden Sie auf der Lehrveranstaltungsseite in BOKUonline.