790357 Up- und downstream-processing

Semester hours
Lecturer (assistant)
Chariyev-Prinz, Farhad , Kasper, Cornelia , Buyel, Johannes
Offered in
Wintersemester 2023/24
Languages of instruction


Upstream processing
Selection of:
- production host
- medium/supplements
- cultivation modes and feeding strategies
- consumables/equipment/disposables
- examples of bioreactors and processes for biotechnological and biopharmaceutical products

Downstream Processing
•Host cell derived impurities (Protein, DNA, endotoxins, critical impurities)
•Flocculation, Precipitation, Crystallization
•Protein refolding
•Removal of impurities and adventitious agents
•Virus/TSE inactivation strategies
•Continuous purification strategies (SMB, CCE, CCC)
•Purification of antibodies, plasmids, viruses, and virus like particles
•Protein stabilisation

Previous knowledge expected

Basics chemical engineering knowledge is requested. This includes basics in heat, mass, momentum transfer, and basics in thermodynamics. Solutions of differential equations (ODE solver). Furthermore basic knowledge in cell culture technology and cell biology is desirable

Objective (expected results of study and acquired competences)

After successful completion of this course, students are able:
-to select suitable medium composition and supplements, choice of production host, selsct suitable reactor, cultivaton mode and feeding strategy a swell as unit operations for a given biotechnological production problem, such as manufacturing of bioactive compounds (eg protein, antibody vaccine)
-to select appropriate quality and in-process control methods and standards for biotechnological manufacturing.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.