790413 Instructional course IIIA - animal cell culture and technology (in Eng.)
- Type
- Exercise course
- Semester hours
- 2
- Lecturer (assistant)
- Kunert, Renate , Marx, Nicolas , Borth, Nicole
- Organisation
- Offered in
- Wintersemester 2023/24
- Languages of instruction
- Englisch
- Content
-
"Isolation and Characterization of Primary Mammalian Cells":
- Isolation of primary cells from different mouse tissues
- Immunofluorescence staining for cell-type specific markers
- Chromosome preparation
- Microscopy
- Discussion and interpretation of results
establishment of recombinant cell lines,
cell analysis for viability, apoptosis, stress resistance and surface marker profiles by flow cytometry and fluorescence microscopy
- Objective (expected results of study and acquired competences)
-
After completion of this course students are familiar with various animal cell cultures and associated advanced technologies. They can outline the individual potential of different animal cell systems for the production of recombinant proteins.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.