790413 Instructional course IIIA - animal cell culture and technology (in Eng.)

Art
Übung
Semesterstunden
2
Vortragende/r (Mitwirkende/r)
Kunert, Renate , Marx, Nicolas , Borth, Nicole
Organisation
Angeboten im Semester
Wintersemester 2023/24
Unterrichts-/ Lehrsprachen
Englisch
Lehrinhalt

Part "Cell culture engineering"
Routine cell culture of CHO cells
- cell density measurement
- calculation of seeding density
- passaging
- calculation of growth rate

CRISPR/Cas9 editing:
- Transfection of CHO cells with plasmids coding for CRISPR/Cas9
- Analysis of transfection efficiency with flow cytometry
- Genotyping of DNA edits
- Staining and flow cytometry analysis of phenotypic effect
- Discussion and interpretation of results


Partr II "Bioprocessing of mammalian cells"
- Assembly and programming of a stirred tank bioreactor
- Sterile connection of periphery
- Medium preparation and inoculation of the bioreactor with recombinant CHO cells
- Daily sampling to determine cell concentration, viability, recombinant protein concentration, nutrient and metabolite concentrations
- Documentation and adjustment of relevant culture parameters e.g. pH, DO etc.
- Data analysis and interpretation
Lehrziel

After completion of this course students are familiar with various animal cell cultures and associated advanced technologies. They can outline the individual potential of different animal cell systems for the production of recombinant proteins.
Noch mehr Informationen zur Lehrveranstaltung, wie Termine oder Informationen zu Prüfungen, usw. finden Sie auf der Lehrveranstaltungsseite in BOKUonline.