790327 Practical course in plant biotechnology

Type: Exercise course
Semester hours: 3
Lecturer (assistant): Maghuly, Fatemeh , Marzban, Gorji , Kapusi, Eszter
Organisation
Offered in: Sommersemester 2025
Languages of instruction: Englisch
Content: Preparation of culture media, working under aseptic conditions; surface sterilization and preparation of the source isolate (tissue); initiation and passaging of callus cultures and suspension cultures, explants; isolation of protoplasts; exercises to study the influence of periphysis, cyclophysis and topophysis on the establishment of cultures, mediated transformation by means of Agrobacterium, GUS assay
Previous knowledge expected: 791111 Plant Biotechnology
Objective (expected results of study and acquired competences): Students will be experienced in the practical implementation of the techniques of in-vitro culture and protein analyses:
+ know how to establish, maintain, propagate different samples of plant tissues in vitro
+ handle the transfer to glass house conditions
+ be able to induce somatic embryogenesis
+ carry out transformation experiments
+ verify the transgenic status of plant material
+ analyse independently plant (fruit ) samples for the presence of allergens

You can find more details like the schedule or information about exams on the course-page in BOKUonline.