790327 Practical course in plant biotechnology (in Eng.)
- Art
- Übung
- Semesterstunden
- 3
- Vortragende/r (Mitwirkende/r)
- Maghuly, Fatemeh , Kapusi, Eszter
- Organisation
- Biotechnologie und Lebensmittelwissenschaften
- Angeboten im Semester
- Sommersemester 2025
- Unterrichts-/ Lehrsprachen
- Englisch
- Lehrinhalt
-
Preparation of culture media and working under aseptic conditions; surface sterilization and preparation of explants (plant tissue); initiation and passaging of callus and suspension cultures; exercises to study the influence of explant orientation (topophysis) and environmental factors on the establishment and growth of cultures; transformation of cultures (e.g., via Agrobacterium or biolistic methods) and evaluation of transformation efficiency by PCR and fluorescence assays.
- Inhaltliche Voraussetzungen (erwartete Kenntnisse)
-
791111 Plant Biotechnology
- Lehrziel
-
Students will be experienced in the practical implementation of the techniques of in-vitro culture and protein analyses:
+ know how to establish, maintain, propagate different samples of plant tissues in vitro
+ handle the transfer to glass house conditions
+ be able to induce somatic embryogenesis
+ carry out transformation experiments
+ verify the transgenic status of plant material
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finden Sie auf der Lehrveranstaltungsseite in BOKUonline.