750410 Instructional course IB - protein design by directed evolution
- Type
- Exercise course
- Semester hours
- 2
- Lecturer (assistant)
- Peterbauer, Clemens Karl , Haltrich, Dietmar , Wozniak-Knopp, Gordana
- Organisation
- Offered in
- Sommersemester 2025
- Languages of instruction
- Englisch
- Content
-
Protein engineering by rational, semi-rational and random methods is an important method in adapting properties of protein. In this practical course two examples, engineering of an enzyme by a semi-rational approach (site-saturation mutagenesis at a selected position with subsequent screening for improved activity in MTP) as well as a random approach (yeast display of a randomly constructed antibody library, a scFC library, and FACS-based screening of improved thermostability) will be given as examples
- Previous knowledge expected
-
basic knowledge in molecular biology and protein / enzyme chemistry
.
- Objective (expected results of study and acquired competences)
-
knowledge on different methods of protein engineering (rational and random approaches to engineer proteins)
.
You can find more details like the schedule or information about exams on the course-page in BOKUonline.