750410 Instructional course IB - protein design by directed evolution (in Eng.)

Art
Übung
Semesterstunden
2
Vortragende/r (Mitwirkende/r)
Peterbauer, Clemens Karl , Haltrich, Dietmar , Wozniak-Knopp, Gordana
Organisation
Angeboten im Semester
Sommersemester 2025
Unterrichts-/ Lehrsprachen
Englisch
Lehrinhalt

Protein engineering by rational, semi-rational and random methods is an important method in adapting properties of protein. In this practical course two examples, engineering of an enzyme by a semi-rational approach (site-saturation mutagenesis at a selected position with subsequent screening for improved activity in MTP) as well as a random approach (yeast display of a randomly constructed antibody library, a scFC library, and FACS-based screening of improved thermostability) will be given as examples
Inhaltliche Voraussetzungen (erwartete Kenntnisse)

Grundkenntnisse in Molekularbiologie und Protein-(Enzym-)chemie
.
Lehrziel

Students who successfully complete this course will be able to:
- compare different methods (directed mutagenesis, random mutagenesis) for protein improvement
- comment on the concept of library design, suitable for a particular aspect of protein evolution
- implement the principle of protein display for the improvement of its properties
- apply the parameters of library construction and selection for its directed evolution
Noch mehr Informationen zur Lehrveranstaltung, wie Termine oder Informationen zu Prüfungen, usw. finden Sie auf der Lehrveranstaltungsseite in BOKUonline.