772328 Methods in protein characterization
- Type
- Lecture and exercise
- Semester hours
- 4
- Lecturer (assistant)
- Organisation
- Biochemistry
- Offered in
- Wintersemester 2024/25
- Languages of instruction
- Englisch
- Content
-
The following topics will be covered:
UNIT 1:
Protein Quality Control with Focus on Differential Scanning Calorimetry
UNIT 2:
Introduction to flow cytometry
UNIT 3:
Affinity, avidity, binding kinetics
UNIT 4:
Cell-based assays for affinity analysis
UNIT 5:
Surface Plasmon Resonance to Determine Affinity and Kinetics of Biomolecular Interactions
UNIT 6:
More Biophysical Techniques to Study Biomolecular Interactions
UNIT 7:
Mass spectrometry (MS) techniques for biomolecules
UNIT 8:
Protein identification via MS
UNIT 9:
Quantification and analysis of posttranslational modifications via MS
HANDS-ON (please see Anmerkung/Note)
- Previous knowledge expected
-
Basic knowledge of biochemistry, in particular protein biochemistry.
- Objective (expected results of study and acquired competences)
-
This course will provide an overview of state-of-the-art techiques to characterize a protein sample in a comprehensive way. One focus will be on the characterization of biomolecular interactions and the available approaches to study them. The course will convey the theoretical concepts and also provide the possibility of hands-on training for selected methods (mass spectrometry, surface plasmon resonance spectroscopy, flow cytometry).
You can find more details like the schedule or information about exams on the course-page in BOKUonline.