772328 Methods in protein characterization (in Eng.)

Art
Vorlesung und Übung
Semesterstunden
4
Vortragende/r (Mitwirkende/r)
Organisation
Biochemie
Angeboten im Semester
Wintersemester 2024/25
Unterrichts-/ Lehrsprachen
Englisch
Lehrinhalt

The following topics will be covered:

UNIT 1:
Protein Quality Control with Focus on Differential Scanning Calorimetry

UNIT 2:
Introduction to flow cytometry

UNIT 3:
Affinity, avidity, binding kinetics

UNIT 4:
Cell-based assays for affinity analysis

UNIT 5:
Surface Plasmon Resonance to Determine Affinity and Kinetics of Biomolecular Interactions

UNIT 6:
More Biophysical Techniques to Study Biomolecular Interactions

UNIT 7:
Mass spectrometry (MS) techniques for biomolecules

UNIT 8:
Protein identification via MS

UNIT 9:
Quantification and analysis of posttranslational modifications via MS

HANDS-ON (please see Anmerkung/Note)
Inhaltliche Voraussetzungen (erwartete Kenntnisse)

Basic knowledge of biochemistry, in particular protein biochemistry.
Lehrziel

This course will provide an overview of state-of-the-art techiques to characterize a protein sample in a comprehensive way. One focus will be on the characterization of biomolecular interactions and the available approaches to study them. The course will convey the theoretical concepts and also provide the possibility of hands-on training for selected methods (mass spectrometry, surface plasmon resonance spectroscopy, flow cytometry).
Noch mehr Informationen zur Lehrveranstaltung, wie Termine oder Informationen zu Prüfungen, usw. finden Sie auf der Lehrveranstaltungsseite in BOKUonline.